一种使用阳离子卟啉对血清中锌进行简单且灵敏的比色测定法。
A simple and sensitive colorimetric assay of zinc in serum using cationic porphyrin.
作者信息
Makino T
机构信息
Department of Clinical Chemistry, Kanagawa Prefectural College of Nursing and Medical Technology, Yokohama, Japan.
出版信息
Clin Chim Acta. 1999 Apr;282(1-2):65-76. doi: 10.1016/s0009-8981(99)00012-1.
A direct colorimetric method is presented for simple and sensitive determination of serum zinc in 0.05-ml samples, using a cationic porphyrin, alpha,beta,gamma,delta-tetrakis(4-N-trimethylaminophenyl) porphine tetratoluenesulfonate salt (ttmapp, epsilon = 41.5 x 10(4) l/mol per cm at 421 nm). 7-Iodo-8-hydroxyquinoline-5-sulfonic acid (Ferron) as an accelerator for the incorporation of zinc into ttmapp was most effective. Interference of iron, copper and conjugated bilirubin in serum can be eliminated in the presence of proteins such as albumin in serum. Within-run and between-run coefficients of variation (CV) were in the ranges of 0.76-3.59 and 2.08-5.20%. A good correlation was observed between this method and atomic absorption spectrometry (AAS).
本文介绍了一种直接比色法，用于简单、灵敏地测定0.05 ml血清样本中的锌，该方法使用阳离子卟啉α,β,γ,δ-四（4-N-三甲基氨基苯基）卟啉四甲苯磺酸盐（ttmapp，在421 nm处的摩尔吸光系数ε = 41.5×10⁴ l/mol·cm）。7-碘-8-羟基喹啉-5-磺酸（Ferron）作为锌掺入ttmapp的促进剂最为有效。血清中铁、铜和结合胆红素的干扰可在血清中存在白蛋白等蛋白质的情况下消除。批内和批间变异系数（CV）分别在0.76 - 3.59%和2.08 - 5.20%范围内。该方法与原子吸收光谱法（AAS）之间具有良好的相关性。
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