Nakagami T, Murakami A, Okisaka S, Ebihara N
Department of Ophthalmology, Hamamatsu University School of Medicine, Shizuoka, Japan.
Jpn J Ophthalmol. 1999 Mar-Apr;43(2):75-9. doi: 10.1016/s0021-5155(98)00069-0.
To investigate the pathogenesis of pterygium.
The number and phenotype of mast cells were examined in excised tissue from 35 pterygia patients and compared with those in normal conjunctival specimens obtained during cataract or other intraocular surgery.
Toluidine blue staining showed that the mean number of mast cells in the pterygia specimens was twice as high as that in the normal conjunctival tissues. Immunohistochemistry with a primary antibody to tryptase, specific for mast cells, also revealed a twofold increase in the mast cell number in the pterygia specimens compared with the normal conjunctival tissues. In the pterygia, more than 94% of the tryptase-positive mast cells were found to express chymase and c-kit. Almost all mast cells in the pterygia were tryptase-positive, chymase-positive mast cells (MC(TC)S). There was no phenotypic difference between the mast cells in the pterygia and those in the normal conjunctival tissues.
The MC(TC)S appear not to be immune system-related and to have functions in angiogenesis and tissue remodeling. The increase in the number of mast cells caused by nonallergic stimulation may contribute to the pathogenesis of pterygium.
研究翼状胬肉的发病机制。
检测35例翼状胬肉患者切除组织中肥大细胞的数量和表型，并与白内障或其他眼内手术中获取的正常结膜标本进行比较。
甲苯胺蓝染色显示，翼状胬肉标本中肥大细胞的平均数量是正常结膜组织中的两倍。用对肥大细胞特异的抗胰蛋白酶原抗体进行免疫组织化学检测也显示，翼状胬肉标本中的肥大细胞数量比正常结膜组织增加了两倍。在翼状胬肉中，发现超过94%的抗胰蛋白酶原阳性肥大细胞表达糜蛋白酶和c-kit。翼状胬肉中几乎所有肥大细胞都是抗胰蛋白酶原阳性、糜蛋白酶阳性的肥大细胞（MC(TC)S）。翼状胬肉中的肥大细胞与正常结膜组织中的肥大细胞在表型上没有差异。
MC(TC)S似乎与免疫系统无关，在血管生成和组织重塑中发挥作用。非过敏性刺激导致的肥大细胞数量增加可能有助于翼状胬肉的发病机制。
