Georgakopoulos Constantinos D, Makri Olga E, Pagoulatos Dionisios, Karamanos Nikolaos K
Department of Ophthalmology, University of Patras, Medical School, Greece.
Biochemistry Laboratory, Department of Chemistry, University of Patras, Greece.
Med Hypothesis Discov Innov Ophthalmol. 2019 Spring;8(1):39-43.
Pterygium is a triangle-shaped fibrovascular hyperplasia of the bulbar conjunctiva on the cornea. The purpose of this study was to analyze Proteoglycans (PGs) by Immunohistochemistry (IHC) in pterygium tissues and to compare the results with normal conjunctiva. Twenty-four patients (14 males) undergoing primary pterygium excision and 17 healthy individuals (10 males), undergoing extracapsular cataract surgery, were included. Pterygium tissues and normal conjunctiva tissues were surgically removed. The tissue sections were fixed in 2% paraformaldehyde and incubated with monoclonal antibodies against PGs anti-mouse IgG. Immunohistochemical study showed stronger expression of keratan sulfate in the stroma of the pterygium compared to normal conjunctiva. An increased expression of heparan sulfate was observed in the epithelial layer and around the pterygium vessels. On the other hand, dermatan sulfate showed an increased expression and localization not only in the sub-epithelial area of the pterygium and normal conjunctiva, yet throughout the stroma of the pterygium. The differences in the expression and localization of the studied extracellular matrix proteoglycans in the pterygium tissue compared to normal conjunctiva may explain the tissue hyperplasia, structure, and the functional properties in pterygium.
翼状胬肉是一种角膜上球结膜的三角形纤维血管增生。本研究的目的是通过免疫组织化学（IHC）分析翼状胬肉组织中的蛋白聚糖（PGs），并将结果与正常结膜进行比较。纳入了24例接受原发性翼状胬肉切除术的患者（14例男性）和17例接受白内障囊外摘除术的健康个体（10例男性）。手术切除翼状胬肉组织和正常结膜组织。将组织切片固定在2%多聚甲醛中，并用抗PGs抗小鼠IgG单克隆抗体孵育。免疫组织化学研究表明，与正常结膜相比，翼状胬肉基质中硫酸角质素的表达更强。在翼状胬肉上皮层和血管周围观察到硫酸乙酰肝素表达增加。另一方面，硫酸皮肤素不仅在翼状胬肉和正常结膜的上皮下区域表达增加且定位改变，而且在整个翼状胬肉基质中均有此现象。与正常结膜相比，翼状胬肉组织中所研究的细胞外基质蛋白聚糖的表达和定位差异可能解释了翼状胬肉的组织增生、结构和功能特性。
