PAX6在发育中的人眼中的表达。
PAX6 expression in the developing human eye.
作者信息
Nishina S, Kohsaka S, Yamaguchi Y, Handa H, Kawakami A, Fujisawa H, Azuma N
机构信息
Department of Ophthalmology, National Children's Hospital, Tokyo, Japan.
出版信息
Br J Ophthalmol. 1999 Jun;83(6):723-7. doi: 10.1136/bjo.83.6.723.
AIMS
To investigate the changes in PAX6 expression in the developing human eye.
METHODS
Six developing human eyes from 6 to 22 weeks' gestation were evaluated. Frozen sections were immunohistochemically stained with monoclonal antibody to chick Pax6 (amino acids 1-223). To verify antibody specificity, western blot analysis was carried out using cell lysates from P19 cells transfected with the human PAX6 gene.
RESULTS
Western blot analysis demonstrated that the antibody reacted to human PAX6 protein. Positive immunostainings for PAX6 were seen in the surface ectoderm, lens vesicle, inner and outer layers of the optic cup, and optic stalk at 6 weeks, and in the corneal epithelia and conjunctiva, lens, and non-pigmented ciliary epithelia from 8 to 22 weeks. In the retina, positive cells were seen in the entire retina from 8 to 10 weeks, and were restricted to the ganglion cell layer and the inner and outer portions of the inner nuclear layer after 21 weeks.
CONCLUSIONS
PAX6 is expressed on the surface and neuroectoderms at an early stage, then in the differentiating cells in the cornea, lens, ciliary body, and retina through development. PAX6 may play a role in determining cell fate in the morphogenesis of various human ocular tissue.
目的
研究发育中的人眼PAX6表达的变化。
方法
评估6例妊娠6至22周的发育中的人眼。冰冻切片用抗鸡Pax6（氨基酸1 - 223）单克隆抗体进行免疫组织化学染色。为验证抗体特异性，使用转染了人PAX6基因的P19细胞的细胞裂解物进行蛋白质印迹分析。
结果
蛋白质印迹分析表明该抗体与人PAX6蛋白发生反应。6周时，在表面外胚层、晶状体泡、视杯内层和外层以及视柄中可见PAX6阳性免疫染色，8至22周时，在角膜上皮、结膜、晶状体和无色素睫状体上皮中可见。在视网膜中，8至10周时在整个视网膜中可见阳性细胞，21周后局限于神经节细胞层以及内核层的内层和外层部分。
结论
PAX6在早期在表面和神经外胚层表达，然后在发育过程中在角膜、晶状体、睫状体和视网膜的分化细胞中表达。PAX6可能在各种人类眼组织形态发生中决定细胞命运方面发挥作用。
Zotero 插件