Lerdthusnee K, Chareonviriyaphap T
National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Bangkok, Thailand.
J Am Mosq Control Assoc. 1999 Mar;15(1):48-52.
Isozyme patterns of 13 field-collected populations of Aedes aegypti from Thailand were compared using starch gel electrophoresis. Three populations were collected before the Bacillus thuringiensis var. israelensis, (B.t.i.) application was initiated. The other 10 populations were collected after the B.t.i. treatment. Results revealed that the number of polymorphic loci were lower in the B.t.i. treated populations as compared to controls. In addition, lower genetic variability was found in populations collected from B.t.i. treated sites (Mae Ka Sa [KS] and Mae Kud Luang [KL] village). These results are most likely due to a genetic bottleneck produced by the B.t.i. treatment. Heterozygosity increased in the months following B.t.i. treatment, probably because of immigration when the control program was withdrawn. However, the anticipated reduction in the expected heterozygosity was only observed in the KS site. This may be due to preexisting low heterozygosity in the KL population. No fixed differences in alleles were detected among the 13 populations.
利用淀粉凝胶电泳比较了从泰国野外采集的13个埃及伊蚊种群的同工酶模式。在苏云金芽孢杆菌以色列变种（B.t.i.）开始应用之前采集了3个种群。其他10个种群是在B.t.i.处理之后采集的。结果显示，与对照相比，经B.t.i.处理的种群中多态位点的数量更少。此外，在从B.t.i.处理地点（湄卡萨[KS]和湄库德銮[KL]村）采集的种群中发现遗传变异性较低。这些结果很可能是由于B.t.i.处理导致的遗传瓶颈。在B.t.i.处理后的几个月里杂合度增加，这可能是因为控制项目撤销后有迁入现象。然而，预期杂合度的降低只在KS地点观察到。这可能是由于KL种群中预先存在较低的杂合度。在这13个种群中未检测到等位基因的固定差异。
