Becker N, Ludwig M
German Mosquito Control Association (KABS), Waldsee.
J Am Mosq Control Assoc. 1993 Jun;9(2):221-4.
In the Upper Rhine Valley (Germany), Bacillus thuringiensis var. israelensis has been widely used against floodwater mosquitoes over an area of approximately 500 km2 for more than 10 years. The susceptibility of larvae of Aedes vexans field populations in 3 untreated (Lake Constance) and 3 treated areas (Upper Rhine Valley) was assessed by means of bioassays with B.t.i. (Bactimos WP, 6,000 AAU/mg), following WHO guidelines. Log-probit analyses and statistical evaluations of the data showed that the LC50 values as well as slopes of bioassays of the larvae deriving from the different areas showed no significant differences. Two populations in the treated area were even more susceptible than populations from the untreated areas. These results have been confirmed by resistance ratios, which were less than one in all tests carried out.
在德国上莱茵河谷地区，苏云金芽孢杆菌以色列变种已在约500平方公里的区域内广泛用于防治洪水蚊，持续时间超过10年。按照世界卫生组织的指导方针，采用含以色列变种苏云金芽孢杆菌（Bactimos WP，6,000国际毒力单位/毫克）的生物测定法，评估了3个未处理区域（康斯坦茨湖）和3个已处理区域（上莱茵河谷）的骚扰伊蚊野外种群幼虫的易感性。对数据进行的对数-概率分析和统计评估表明，来自不同区域的幼虫生物测定的半数致死浓度（LC50）值和斜率均无显著差异。已处理区域的两个种群甚至比未处理区域的种群更易感。所有测试的抗性比率均小于1，这证实了上述结果。
