免疫组织化学染色不同评分系统的比较
Comparison of different scoring systems for immunohistochemical staining.
作者信息
Adams E J, Green J A, Clark A H, Youngson J H
机构信息
Department of Medicine, University of Liverpool, UK.
出版信息
J Clin Pathol. 1999 Jan;52(1):75-7. doi: 10.1136/jcp.52.1.75.
AIM
To carry out an objective assessment of two systems of scoring immunohistochemical staining, evaluating interobserver and intraobserver error.
METHODS
92 cervical tumours underwent immunohistochemical staining for p53 and epidermal growth factor receptor. Staining was assessed using two methods: a standard 4 point scale and a descriptive method, performed by three observers. Interobserver and intraobserver error were assessed for both scoring methods.
RESULTS
In terms of interobserver error between three observers, no difference was found between a simple 4 point scale method of evaluation and the use of a highly circumscribed method. In all evaluations, interobserver error was scored as moderate (kappa w 0.48-0.49). However, evaluation of immunohistochemical staining by a panel of observers led to a marked improvement in the interobserver error scores (kappa w 0.63).
CONCLUSIONS
There should be standardisation of immunohistochemical staining and scoring methods. More attention should be paid to measurement of interobserver and intraobserver error in studies. Use of a panel of tissue control slides and consensus scoring by several observers can lead to improvement in reproducibility.
目的
对两种免疫组织化学染色评分系统进行客观评估，评价观察者间和观察者内误差。
方法
92例宫颈肿瘤进行了p53和表皮生长因子受体的免疫组织化学染色。采用两种方法评估染色：标准的4分制和描述性方法，由三名观察者进行。对两种评分方法均评估了观察者间和观察者内误差。
结果
在三名观察者之间的观察者间误差方面，简单的4分制评估方法与使用高度限定的方法之间未发现差异。在所有评估中，观察者间误差评分为中度（kappa值为0.48 - 0.49）。然而，由一组观察者对免疫组织化学染色进行评估导致观察者间误差评分有显著改善（kappa值为0.63）。
结论
免疫组织化学染色和评分方法应标准化。在研究中应更多关注观察者间和观察者内误差的测量。使用一组组织对照切片并由多名观察者进行共识评分可提高可重复性。
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