Baum M, Klöpping-Menke K, Müller-Steinhardt M, Liesen H, Kirchner H
Institute of Sports Medicine, University of Paderborn, Germany.
Eur J Appl Physiol Occup Physiol. 1999 May;79(6):500-3. doi: 10.1007/s004210050544.
The aim of the study was to investigate whether a regular moderate endurance exercise programme influenced the in vitro cytokine synthesis by stimulated whole blood cultures. To this end, eight healthy subjects exercised moderately by running for 3-5 h a week over a period of 12 weeks, whilst seven other healthy subjects served as the control group. The intensity of the exercise was determined by lactic acid concentrations in the blood which were maintained between 1.8 and 2.5 mmol x l(-1). Over the period of training the running velocity producing the 4 mmol x l(-1) lactic acid threshold increased from 2.86 (SD 0.83) m x s(-1) to 3.06+/-0.79 m x s(-1) (P < or = 0.008). Blood samples were taken at rest before and after the training programme. The following blood parameters were determined: leucocyte count, differential leucocyte count, lymphocyte subpopulations [CD14 positive (+)/CD45+, CD4+/ CD25+, CD8+, CD16+/CD122+]. Whole blood cultures were stimulated with lipopolysaccarides [interleukin (IL)-1 beta and IL-6] and staphylococcal enterotoxin B [IL-2, soluble interleukin 2 receptor (sIL2-R) and interferon (IFN)-gamma]. Cytokine concentrations in the supernatants were measured using an enzyme-linked immunosorbent assay. The white blood cell count, differential leucocyte count, lymphocyte subset distribution and the expression of the CD25 and CD122 antigen on lymphocytes were unchanged by training. After the training programme the IL-1 beta production changed significantly [1496 (SD 264) pg ml(-1) before, compared to 2127 (SD 672) pg ml(-1) after training, P < or = 0.008]. In the control group these parameters remained unchanged. With respect to changes in the values in both groups the syntheses of IL-1 beta (P < or = 0.023) and IL-6 (P < or = 0.021) were significantly higher after regular training. The syntheses of IL-2, sIL-2 and INF-gamma were not significantly influenced. Regular endurance exercise influenced the in vitro production of monocyte derived cytokines, while the effect of exercise on the cytokines synthesized by T-cells appeared to be of lesser importance.
本研究的目的是调查定期适度耐力运动计划是否会影响刺激全血培养物的体外细胞因子合成。为此，8名健康受试者在12周的时间里每周进行3 - 5小时的适度跑步运动，而另外7名健康受试者作为对照组。运动强度通过维持在1.8至2.5 mmol·L⁻¹之间的血液乳酸浓度来确定。在训练期间，产生4 mmol·L⁻¹乳酸阈值的跑步速度从2.86（标准差0.83）m·s⁻¹增加到3.06±0.79 m·s⁻¹（P≤0.008）。在训练计划前后的静息状态下采集血样。测定以下血液参数：白细胞计数、白细胞分类计数、淋巴细胞亚群[CD14阳性（+）/CD45 +、CD4 + /CD25 +、CD8 +、CD16 + /CD122 +]。用脂多糖[白细胞介素（IL）-1β和IL-6]和葡萄球菌肠毒素B[IL-2、可溶性白细胞介素2受体（sIL2-R）和干扰素（IFN）-γ]刺激全血培养物。使用酶联免疫吸附测定法测量上清液中的细胞因子浓度。训练对白细胞计数、白细胞分类计数、淋巴细胞亚群分布以及淋巴细胞上CD25和CD122抗原的表达没有影响。训练计划后，IL-1β的产生有显著变化[训练前为1496（标准差264）pg/ml，训练后为2127（标准差672）pg/ml，P≤0.008]。在对照组中这些参数保持不变。就两组数值的变化而言，定期训练后IL-1β（P≤0.023）和IL-6（P≤0.021）的合成显著更高。IL-2、sIL-2和INF-γ的合成没有受到显著影响。定期耐力运动影响单核细胞衍生细胞因子的体外产生，而运动对T细胞合成的细胞因子的影响似乎较小。
