Ding Min, Zhao Chaoxian, Li Yan, Liu Xiaoxia, Wang Xueling, Liu Fengli, Wang Jian, Xiong Nanyan, Song Yonghong, Xu Yanli
Central Laboratory, Medical College, Hebei University of Engineering, Handan, Hebei 056038, P.R. China.
Department of Biochemistry, Medical College, Hebei University of Engineering, Handan, Hebei 056002, P.R. China.
Exp Ther Med. 2020 Aug;20(2):1782-1788. doi: 10.3892/etm.2020.8799. Epub 2020 May 27.
Changes in the levels of interleukin-1β (IL-1β), cortisol and chromogranin A (CgA) in saliva of subjects with occupational fatigue were investigated. Doctors in the Emergency Department were selected as research subjects. Saliva was collected before work (after full rest) and after work (≥24 h). Electroencephalogram (EEG) was performed. Enzyme-linked immunosorbent assay (ELISA) was used to detect the levels of IL-1β, cortisol and CgA in saliva. In order to obtain permission for human specimens, the study was approved by the Ethics Committee of the Affiliated Hospital of Hebei University of Engineering and registered for clinical trials (registration no. ChiC-TR-DCD-14005746). As there were only 4 subjects in this study without fatigue waves in EEG, and the number of these subjects was not sufficient to constitute a control group, the comparison of the contents of IL-1β, cortisol and CgA of all subjects before and after working for 18 h was just a confirmation of the statistical results of 43 cases with fatigue waves in the EEG. According to the results, there was no change in the contents of IL-1β and cortisol in the saliva of subjects with occupational fatigue before and after fatigue, whereas, there was a significant change in the content of CgA before and after fatigue. However, there was no correlation between the content of CgA and fatigue. The results of the present study revealed that IL-1β, cortisol and CgA indicators are not suitable diagnostic markers for occupational fatigue.
对职业疲劳受试者唾液中白细胞介素-1β（IL-1β）、皮质醇和嗜铬粒蛋白A（CgA）水平的变化进行了研究。选取急诊科医生作为研究对象。在工作前（充分休息后）和工作后（≥24小时）采集唾液。进行脑电图（EEG）检查。采用酶联免疫吸附测定（ELISA）法检测唾液中IL-1β、皮质醇和CgA的水平。为获得使用人体标本的许可，本研究经河北工程大学附属医院伦理委员会批准并注册为临床试验（注册号：ChiC-TR-DCD-14005746）。由于本研究中仅有4名受试者脑电图无疲劳波，且这些受试者数量不足以构成对照组，因此对所有受试者工作18小时前后IL-1β、皮质醇和CgA含量的比较只是对43例脑电图有疲劳波患者统计结果的确认。结果显示，职业疲劳受试者疲劳前后唾液中IL-1β和皮质醇含量无变化，而疲劳前后CgA含量有显著变化。然而，CgA含量与疲劳之间无相关性。本研究结果表明，IL-1β、皮质醇和CgA指标不是职业疲劳的合适诊断标志物。
