利用大肠杆菌生物素缺失突变体对生物素浓度进行生物测定。
Bioassay of biotin concentration with a Escherichia coli bio deletion mutant.
作者信息
Hwang S Y, Su V, Farh L, Shiuan D
机构信息
Department of Biological Sciences, National Sun Yat-Sen University, Kaohsiung, Taiwan.
出版信息
J Biochem Biophys Methods. 1999 Feb 25;39(1-2):111-4. doi: 10.1016/s0165-022x(98)00012-8.
Abstract
Biotin concentration was determined unequivocally with the E. coli bio mutant. The results demonstrate that this simple and efficient method can determine biotin concentration in the range of 10 pg to 50 ng/ml. The present method can also clearly distinguish biotin from its precursor and analog, dethiobiotin.
摘要
利用大肠杆菌生物突变体明确测定了生物素浓度。结果表明，这种简单有效的方法能够测定浓度范围在10皮克至50纳克/毫升的生物素。本方法还能清晰地区分生物素与其前体及类似物——脱硫生物素。
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