Hwang S Y, Su V, Farh L, Shiuan D
Department of Biological Sciences, National Sun Yat-Sen University, Kaohsiung, Taiwan.
J Biochem Biophys Methods. 1999 Feb 25;39(1-2):111-4. doi: 10.1016/s0165-022x(98)00012-8.
Biotin concentration was determined unequivocally with the E. coli bio mutant. The results demonstrate that this simple and efficient method can determine biotin concentration in the range of 10 pg to 50 ng/ml. The present method can also clearly distinguish biotin from its precursor and analog, dethiobiotin.
利用大肠杆菌生物突变体明确测定了生物素浓度。结果表明，这种简单有效的方法能够测定浓度范围在10皮克至50纳克/毫升的生物素。本方法还能清晰地区分生物素与其前体及类似物——脱硫生物素。
