Eisenberg M A, Krell K
J Bacteriol. 1969 Jun;98(3):1227-31. doi: 10.1128/jb.98.3.1227-1231.1969.
The synthesis of desthiobiotin from 7,8-diaminopelargonic acid (DAP) was demonstrated in resting cell suspensions of Escherichia coli K-12 bioA mutants under conditions in which the biotin locus was derepressed. The biosynthetically formed desthiobiotin was identified by chromatography, electrophoresis, and by its ability to support the growth of yeast and those E. coli biotin auxotrophs that are blocked earlier in the biotin pathway. Optimal conditions for desthiobiotin synthesis were determined. Desthiobiotin synthetase activity was repressed 67% when partially derepressed resting cells were incubated in the presence of 3 ng of biotin per ml. Serine, bicarbonate, and glucose stimulated desthiobiotin synthesis apparently by acting as sources of CO(2). The results of this study are consistent with an earlier postulated pathway for biotin biosynthesis in E. coli: pimelic acid --> 7-oxo-8-aminopelargonic acid --> DAP --> desthiobiotin --> biotin.
在生物素基因座去阻遏的条件下，在大肠杆菌K-12 bioA突变体的静息细胞悬液中证明了由7,8-二氨基壬酸（DAP）合成脱硫生物素。通过色谱法、电泳以及其支持酵母和那些在生物素途径中较早受阻的大肠杆菌生物素营养缺陷型生长的能力来鉴定生物合成形成的脱硫生物素。确定了脱硫生物素合成的最佳条件。当在每毫升含有3 ng生物素的情况下孵育部分去阻遏的静息细胞时，脱硫生物素合成酶活性被抑制了67%。丝氨酸、碳酸氢盐和葡萄糖显然通过作为CO₂的来源刺激脱硫生物素的合成。本研究结果与早期推测的大肠杆菌生物素生物合成途径一致：庚二酸→7-氧代-8-氨基壬酸→DAP→脱硫生物素→生物素。
