Pai C H, Lichstein H C
J Bacteriol. 1967 Dec;94(6):1930-3. doi: 10.1128/jb.94.6.1930-1933.1967.
Biotin auxotrophs were isolated from Escherichia coli K-12. One of the mutants was unable to grow on desthiobiotin and accumulated a large amount of a vitamer in medium when growing on an optimal concentration of biotin. The production of the vitamer was inhibited in the presence of an excess amount of biotin. The vitamer was identified as desthiobiotin on the basis of biological activities, avidin combinability, and chromatographic characteristics. The mutant lacked the ability to convert desthiobiotin to biotin. These results further support the hypothesis that desthiobiotin is a normal intermediate in the biosynthesis of biotin in E. coli.
从大肠杆菌K-12中分离出生物素营养缺陷型菌株。其中一个突变体不能在脱硫生物素上生长，并且在以最佳浓度生物素培养时在培养基中积累了大量的维生素原。在过量生物素存在的情况下，维生素原的产生受到抑制。根据生物活性、抗生物素蛋白结合能力和色谱特征，该维生素原被鉴定为脱硫生物素。该突变体缺乏将脱硫生物素转化为生物素的能力。这些结果进一步支持了脱硫生物素是大肠杆菌生物素生物合成正常中间体的假说。
