Schmidt M F, Schwarz R T, Scholtissek C
Eur J Biochem. 1976 Nov 1;70(1):55-62. doi: 10.1111/j.1432-1033.1976.tb10955.x.
The predominant effect of 2-deoxy-D-glucose on chick embryo cells infected with Semliki Forest virus is an interference with glycosylation of virus-specific glycoproteins; this results in a block of synthesis of infectious virus. Incorporation of radioactive mannose is blocked severely in the presence of 2-deoxyglucose in the cultural medium although it is readily phosphorylated and subsequently activated by GTP to yield GDP-mannose, which accumulates under these conditions. The intracellular concentrations of GDP-mannose and UDP-N-acetyl-D-hexosamine are not reduced in the presence of the inhibitor. An equimolar concentration of mannose in the cultural medium competes with the inhibitory effect of the deoxysugar and drops the cellular pool of GDP-2-deoxy-D-glucose below the level of detection, at the same time restoring the synthesis of infectious virus. When the intracellular concentration of UDP-2-deoxyglucose is reduced by addition of glucose into the cultural medium the inhibition of virus synthesis by the deoxysugar and the concentration of GDP-2-deoxyglucose within the cells remain near to the values when the inhibitor is present alone. It is concluded that among the metabolites of 2-deoxyglucose which occur in vivo after addition of 2-deoxyglucose to the culture medium, GDP-2-deoxyglucose is the agent responsible for inhibition of glycosylation of viral glycoproteins.
2-脱氧-D-葡萄糖对感染辛德毕斯病毒的鸡胚细胞的主要作用是干扰病毒特异性糖蛋白的糖基化；这导致传染性病毒合成受阻。尽管2-脱氧葡萄糖在培养基中容易被磷酸化并随后被GTP激活生成GDP-甘露糖，且在这些条件下会积累，但放射性甘露糖的掺入在2-脱氧葡萄糖存在时会被严重阻断。在抑制剂存在的情况下，GDP-甘露糖和UDP-N-乙酰-D-己糖胺的细胞内浓度并未降低。培养基中与2-脱氧葡萄糖等摩尔浓度的甘露糖可与脱氧糖的抑制作用竞争，并使细胞内GDP-2-脱氧-D-葡萄糖池降至检测水平以下，同时恢复传染性病毒的合成。当通过向培养基中添加葡萄糖来降低细胞内UDP-2-脱氧葡萄糖的浓度时，脱氧糖对病毒合成的抑制作用以及细胞内GDP-2-脱氧葡萄糖的浓度仍接近仅存在抑制剂时的值。得出的结论是，在向培养基中添加2-脱氧葡萄糖后体内出现的2-脱氧葡萄糖代谢产物中，GDP-2-脱氧葡萄糖是负责抑制病毒糖蛋白糖基化的物质。
