Yanaura S, Funada K, Abe Y, Hosokawa T
Nihon Yakurigaku Zasshi. 1976 Sep;72(6):745-52.
In the present study, the learned behavior caused by hypothalamic electrical stimulation was examined in order to determine the effects of psychotropic drugs. Subjects were albino male rabbits with electrodes chronically implanted in the hypothalamic area. A shuttle box, which was adjusted for behavioral pharmacological estimation of drugs in rabbits, was used. A buzzer sound (85dB) and electrical stimulation of hypothalamus (100 HZ, 1 msec, 1.2-2.0V) were used as the conditional stimulation (CS) and unconditional stimulation (UCS), respectively. The same animal was trained in habituation to a buzzer sound as the CS. For avoidance conditioning in a two-compartment situation, the animal was placed in a shuttle box divided by a hurdle situated at the middle of two-compartments. After the CS was presented for 10 sec, the UCS was given. The animals were subjected to 15 conditioning trials per day. The avoidance and escape behavior model became as distinct by hypothalamic stimulation as by UCS. After termination of the experiments, extinction trials were carried out after which the animals were sacrificed, and localization of the stimulating electrodes was determined histologically.
在本研究中，对下丘脑电刺激引起的习得行为进行了检查，以确定精神药物的作用。实验对象是长期在下丘脑区域植入电极的白化雄性兔子。使用了一个经过调整以用于兔子药物行为药理学评估的穿梭箱。分别使用蜂鸣声（85分贝）和下丘脑电刺激（100赫兹，1毫秒，1.2 - 2.0伏）作为条件刺激（CS）和非条件刺激（UCS）。对同一只动物进行训练，使其习惯将蜂鸣声作为条件刺激。为了在两室环境中进行回避条件反射训练，将动物放置在一个由位于两室中间的隔板隔开的穿梭箱中。在呈现条件刺激10秒后，给予非条件刺激。动物每天接受15次条件反射试验。下丘脑刺激引起的回避和逃避行为模式与非条件刺激引起的一样明显。实验结束后，进行消退试验，之后处死动物，并通过组织学方法确定刺激电极的位置。
