Klesius P H, Kramer T T, Swann A I, Christenberry C C
Am J Vet Res. 1978 May;39(5):883-6.
Cell-mediated immunity to Brucella abortus S19 vaccine was measured in young heifers by the microassay for stimulation of protein synthesis (SPS) with [3H]leucine and the skin test for delayed hypersensitivity. Brucella melitensis protein allergen and a crude B abortus S19-soluble antigen were compared in the SPS test. The SPS test was negative in 5 unvaccinated heifers and strongly positive in 3 twice-vaccinated steers. However, the SPS test was positive only in 13 of 30 S19-vaccinated heifers and the delayed hypersensitivity in 9 of 29 S19-vaccinated heifers. The 2 tests gave good agreement. Vaccination-induced residual antibody titers were partly correlated with the outcome of the tests used to measure cell-mediated immunity.
通过用[3H]亮氨酸刺激蛋白质合成的微量测定法（SPS）和迟发型超敏反应皮肤试验，在年轻小母牛中检测了对流产布鲁氏菌S19疫苗的细胞介导免疫。在SPS试验中比较了羊种布鲁氏菌蛋白变应原和流产布鲁氏菌S19粗可溶性抗原。5头未接种疫苗的小母牛的SPS试验为阴性，3头两次接种疫苗的公牛的SPS试验为强阳性。然而，在30头接种S19疫苗的小母牛中，只有13头的SPS试验呈阳性，在29头接种S19疫苗的小母牛中，只有9头有迟发型超敏反应。这两种试验结果吻合良好。疫苗接种诱导的残留抗体滴度与用于测量细胞介导免疫的试验结果部分相关。
