通过Percoll密度梯度离心法分离的刚地弓形虫猫肠上皮阶段的感染性。

Infectivity of feline enteroepithelial stages of Toxoplasma gondii isolated by Percoll-density gradient centrifugation.

作者信息

Omata Y, Kawano T, Ohsawa T, Sugaya S, Satake M, Isamida T, Koyama T, Taka A, Miyazawa K, Takagi M, Saito A, Toyoda Y

机构信息

Department of Veterinary Physiology, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.

出版信息

Vet Parasitol. 1999 Apr 12;82(3):211-5. doi: 10.1016/s0304-4017(99)00019-9.

DOI:10.1016/s0304-4017(99)00019-9

PMID:10348100

Abstract

The infectivity of the feline enteroepithelial stages of Toxoplasma gondii isolated by Percoll-density gradient centrifugation was examined by the trypan blue dye exclusion method by assaying their penetration into feline fibroblast cells in vitro and by inoculation of the intestinal mucosa of cats. A large population of the parasites showed trypan blue dye exclusion activity. When feline fibroblast cells were inoculated with feline enteroepithelial stage parasites, no intracellular parasites were found 18 h post-inoculation. Kittens inoculated intraduodenally with 2 x 10(6) feline enteroepithelial stage parasites shed oocysts between 2 and 8 days post-inoculation. These results indicate that the isolated feline enteroepithelial stage parasites display infectivity towards enterocytes of cats and are capable of gametogenesis.

摘要

采用台盼蓝染料排除法，通过检测体外弓形虫猫肠上皮阶段虫体对猫成纤维细胞的侵入以及接种猫的肠黏膜，对经Percoll密度梯度离心分离得到的弓形虫猫肠上皮阶段虫体的感染性进行了检测。大量寄生虫表现出台盼蓝染料排除活性。用猫肠上皮阶段虫体接种猫成纤维细胞后，接种18小时未发现细胞内寄生虫。经十二指肠接种2×10⁶个猫肠上皮阶段虫体的小猫在接种后2至8天排出卵囊。这些结果表明，分离得到的猫肠上皮阶段虫体对猫的肠细胞具有感染性，并且能够进行配子生殖。