Koyama T, Shimada S, Ohsawa T, Omata Y, Xuan X, Inoue N, Mikami T, Saito A
Department of Basic Veterinary Science, United Graduate School of Veterinary Sciences, Gifu University, Japan.
J Vet Med Sci. 2000 Oct;62(10):1089-92. doi: 10.1292/jvms.62.1089.
In an investigation aimed to identify Toxoplasma gondii antigens expressed in feline enteroepithelial-stages parasites, a cDNA library was constructed and fourteen positive clones were isolated by immunoscreening using sera from cats immunized with feline enteroepithelial-stages parasites. By DNA sequence homology analysis, these fourteen isolated clones were classified into four groups: hypoxanthine-guanine phosphoribosyl transferase (HGPRT) cDNA, heat shock protein 70 (HSP70) cDNA, 14-3-3 protein homologue cDNA, and cDNA encoding an unknown product. In an indirect immunofluorescence antibody test, sera from mice immunized with the recombinant protein encoded by the cDNA for HGPRT, HSP70, 14-3-3 protein, or the unknown product each showed a relatively high level of immunoreactivity with feline enteroepithelial-stages parasites.
在一项旨在鉴定猫肠道上皮阶段寄生虫中表达的弓形虫抗原的研究中，构建了一个cDNA文库，并使用用猫肠道上皮阶段寄生虫免疫的猫的血清通过免疫筛选分离出14个阳性克隆。通过DNA序列同源性分析，这14个分离的克隆被分为四组：次黄嘌呤-鸟嘌呤磷酸核糖转移酶（HGPRT）cDNA、热休克蛋白70（HSP70）cDNA、14-3-3蛋白同源物cDNA以及编码未知产物的cDNA。在间接免疫荧光抗体试验中，用HGPRT、HSP70、14-3-3蛋白或未知产物的cDNA编码的重组蛋白免疫的小鼠血清，与猫肠道上皮阶段寄生虫均显示出相对较高水平的免疫反应性。
