Omata Y, Taka A, Terada K, Koyama T, Kanda M, Saito A, Dubey J P
Department of Veterinary Physiology, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.
Parasitol Res. 1997;83(6):574-7. doi: 10.1007/s004360050300.
A method for isolation of enteroepithelial stages of Toxoplasma gondii from the intestinal mucosa of experimentally infected cats was developed using Percoll density-gradient centrifugation. Gamonts and merozoites were obtained essentially free of host-cell debris. A recovery rate of nearly 30% of the parasites in the original preparations was obtained by this method. Merozoites were separated from gamonts by filtration through a 3-micron polycarbonate filter.
利用Percoll密度梯度离心法，开发了一种从实验感染猫的肠黏膜中分离刚地弓形虫肠上皮阶段的方法。获得的配子体和裂殖子基本不含宿主细胞碎片。通过该方法，原始制剂中近30%的寄生虫得以回收。通过3微米聚碳酸酯滤器过滤，将裂殖子与配子体分离。
