鱼肉中1,3 - 二乙基 - 2 - 硫代巴比妥酸 - 丙二醛加合物的高效液相色谱测定

High-performance liquid chromatographic determination of 1,3-diethyl-2-thiobarbituric acid--malonaldehyde adduct in fish meat.

作者信息

Sakai T, Habiro A, Kawahara S

机构信息

Faculty of Agriculture, Miyazaki University, Japan.

出版信息

J Chromatogr B Biomed Sci Appl. 1999 Apr 16;726(1-2):313-6. doi: 10.1016/s0378-4347(99)00060-2.

DOI:10.1016/s0378-4347(99)00060-2

PMID:10348202

Abstract

The reaction conditions of 1,3-diethyl-2-thiobarbituric acid (DETBA)-malonaldehyde (MA) adduct formation were examined in order to analyze MA in fish tissue by high-performance liquid chromatography. A reaction mixture containing 4 mM butyl hydroxytoluene was heated at 100 degrees C for 150 min and the DETBA-MA adduct formed was separated by a Inertsil ODS column for 20 min. The detection limit was 5 pmol.

摘要

为了通过高效液相色谱法分析鱼类组织中的丙二醛（MA），研究了1,3 - 二乙基 - 2 - 硫代巴比妥酸（DETBA）与丙二醛（MA）加合物的反应条件。将含有4 mM丁基羟基甲苯的反应混合物在100℃下加热150分钟，形成的DETBA - MA加合物通过Inertsil ODS柱分离20分钟。检测限为5皮摩尔。