Csallany A S, Der Guan M, Manwaring J D, Addis P B
Anal Biochem. 1984 Nov 1;142(2):277-83. doi: 10.1016/0003-2697(84)90465-2.
A high-performance liquid chromatographic method was developed for the quantification of free malonaldehyde (MA) in tissues. HPLC separation was performed using a TSK G1000 PW column (7.5-mm i.d. X 30 cm) with a mobile phase of 0.1 M Na3PO4 buffer, pH 8.0, at a flow rate of 0.6 ml/min. The eluant was monitored at 267 nm. Free MA in the tissue sample was separated and quantified in approximately 50 min. The lowest amount of MA that can be determined by this HPLC technique is approximately 1 ng per injection. This method was successfully applied to rat liver and beef, pork, and chicken muscle and was compared to the thiobarbituric acid (TBA) test. It was found to be more sensitive, accurate, and specific for the determination of free MA than the TBA method.
建立了一种高效液相色谱法用于定量测定组织中的游离丙二醛（MA）。使用TSK G1000 PW柱（内径7.5 mm×30 cm）进行HPLC分离，流动相为0.1 M Na3PO4缓冲液，pH 8.0，流速为0.6 ml/min。在267 nm处监测洗脱液。组织样品中的游离MA在约50分钟内被分离并定量。该HPLC技术能够测定的最低MA量约为每次进样1 ng。该方法成功应用于大鼠肝脏以及牛肉、猪肉和鸡肉肌肉，并与硫代巴比妥酸（TBA）试验进行了比较。结果发现，与TBA法相比，该方法在测定游离MA时更灵敏、准确且特异。
