肉类及肉类产品中的脂质过氧化过程：改良的 2-硫代巴比妥酸分光光度法和反相高效液相色谱法测定丙二醛的比较研究。
Lipid Peroxidation Process in Meat and Meat Products: A Comparison Study of Malondialdehyde Determination between Modified 2-Thiobarbituric Acid Spectrophotometric Method and Reverse-Phase High-Performance Liquid Chromatography.
机构信息
Department of Food Hygiene and Technology, University of Veterinary Medicine and Pharmacy in Košice, 041 81 Košice, Slovakia.
Department of Chemistry, Biochemistry and Biophysics, University of Veterinary Medicine and Pharmacy in Košice, 041 81 Košice, Slovakia.
出版信息
Molecules. 2017 Nov 16;22(11):1988. doi: 10.3390/molecules22111988.
The aim of this work was to compare the methods of malondialdehyde detection, as the main secondary product of the lipid peroxidation process, in meat and meat products. Malondialdehyde measurements were performed by two modified methods, the 2-thiobarbituric acid spectrophotometric method and the reverse-phase high-performance liquid chromatography in raw, mechanically-deboned chicken meat and in manufactured frankfurters. The malondialdehyde concentrations measured by the 2-thiobarbituric acid spectrophotometric method were found to be overestimated by more than 25% in raw meat and more than 27% in frankfurters in comparison to the results of reverse-phase high-performance liquid chromatography ( < 0.05). The achieved results showed that the presented modified reverse-phase high-performance liquid chromatography method was more applicable and more accurate for the quantification of malondialdehyde in samples of meat and meat products.
本工作旨在比较丙二醛（脂质过氧化过程中的主要次级产物）检测方法，丙二醛检测方法分别采用改良的 2-硫代巴比妥酸分光光度法和反相高效液相色谱法，用于检测生的、机械去骨鸡肉和加工的法兰克福香肠中的丙二醛。与反相高效液相色谱法（<0.05）的结果相比，2-硫代巴比妥酸分光光度法测定的生肉和法兰克福香肠中的丙二醛浓度分别高估了 25%以上和 27%以上。研究结果表明，所提出的改良反相高效液相色谱法更适用于肉和肉产品样品中丙二醛的定量分析，且更准确。
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