de Koker Theodorus H, Mozuch Michael D, Cullen Daniel, Gaskell Jill, Kersten Philip J
Forest Products Laboratory, Forest Service, U.S. Department of Agriculture, 1 Gifford Pinchot Dr., Madison, WI 53726-2398, USA.
Appl Environ Microbiol. 2004 Oct;70(10):5794-800. doi: 10.1128/AEM.70.10.5794-5800.2004.
Pyranose 2-oxidase (POX) was recovered from Phanerochaete chrysosporium BKM-F-1767 solid substrate culture using mild extraction conditions and was purified. (13)C-nuclear magnetic resonance confirmed production of d-arabino-hexos-2-ulose (glucosone) from d-glucose with the oxidase. Peptide fingerprints generated by liquid chromatography-tandem mass spectrometry of tryptic digests and analysis of the corresponding cDNA revealed a structurally unusual sequence for the P. chrysosporium POX. Relatively high levels of pox transcript were detected under carbon-starved culture conditions but not under nutrient sufficiency. This regulation pattern is similar to that observed for lignin peroxidases, manganese peroxidases, and glyoxal oxidase of P. chrysosporium, supporting evidence that POX has a role in lignocellulose degradation.
采用温和的提取条件从黄孢原毛平革菌BKM-F-1767固体基质培养物中回收并纯化了吡喃糖2-氧化酶（POX）。碳-13核磁共振证实该氧化酶可将D-葡萄糖转化为D-阿拉伯己糖-2-酮糖（葡糖酮）。通过对胰蛋白酶消化产物进行液相色谱-串联质谱分析以及对相应cDNA进行分析所产生的肽指纹图谱揭示了黄孢原毛平革菌POX的一个结构异常的序列。在碳饥饿培养条件下检测到相对高水平的pox转录本，但在营养充足条件下未检测到。这种调控模式与在黄孢原毛平革菌的木质素过氧化物酶、锰过氧化物酶和乙二醛氧化酶中观察到的模式相似，这支持了POX在木质纤维素降解中起作用的证据。
