从黄孢原毛平革菌中分离纯化吡喃糖2-氧化酶及其基因结构与调控特性研究
Isolation and purification of pyranose 2-oxidase from Phanerochaete chrysosporium and characterization of gene structure and regulation.
作者信息
de Koker Theodorus H, Mozuch Michael D, Cullen Daniel, Gaskell Jill, Kersten Philip J
机构信息
Forest Products Laboratory, Forest Service, U.S. Department of Agriculture, 1 Gifford Pinchot Dr., Madison, WI 53726-2398, USA.
出版信息
Appl Environ Microbiol. 2004 Oct;70(10):5794-800. doi: 10.1128/AEM.70.10.5794-5800.2004.
Abstract
Pyranose 2-oxidase (POX) was recovered from Phanerochaete chrysosporium BKM-F-1767 solid substrate culture using mild extraction conditions and was purified. (13)C-nuclear magnetic resonance confirmed production of d-arabino-hexos-2-ulose (glucosone) from d-glucose with the oxidase. Peptide fingerprints generated by liquid chromatography-tandem mass spectrometry of tryptic digests and analysis of the corresponding cDNA revealed a structurally unusual sequence for the P. chrysosporium POX. Relatively high levels of pox transcript were detected under carbon-starved culture conditions but not under nutrient sufficiency. This regulation pattern is similar to that observed for lignin peroxidases, manganese peroxidases, and glyoxal oxidase of P. chrysosporium, supporting evidence that POX has a role in lignocellulose degradation.
摘要
采用温和的提取条件从黄孢原毛平革菌BKM-F-1767固体基质培养物中回收并纯化了吡喃糖2-氧化酶（POX）。碳-13核磁共振证实该氧化酶可将D-葡萄糖转化为D-阿拉伯己糖-2-酮糖（葡糖酮）。通过对胰蛋白酶消化产物进行液相色谱-串联质谱分析以及对相应cDNA进行分析所产生的肽指纹图谱揭示了黄孢原毛平革菌POX的一个结构异常的序列。在碳饥饿培养条件下检测到相对高水平的pox转录本，但在营养充足条件下未检测到。这种调控模式与在黄孢原毛平革菌的木质素过氧化物酶、锰过氧化物酶和乙二醛氧化酶中观察到的模式相似，这支持了POX在木质纤维素降解中起作用的证据。
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本文引用的文献
1
Purification by Immunoaffinity Chromatography, Characterization, and Structural Analysis of a Thermostable Pyranose Oxidase from the White Rot Fungus Phlebiopsis gigantea.免疫亲和层析纯化、特性分析及白腐真菌嗜热吡喃糖酸酶的结构分析。
Appl Environ Microbiol. 1996 Jul;62(7):2586-92. doi: 10.1128/aem.62.7.2586-2592.1996.
2
Pyranose Oxidase, a Major Source of H(2)O(2) during Wood Degradation by Phanerochaete chrysosporium, Trametes versicolor, and Oudemansiella mucida.漆酶氧化吡喃糖，白腐真菌、绒泡菌和栓菌降解木材过程中产生 H(2)O(2)的主要来源。
Appl Environ Microbiol. 1994 Jul;60(7):2524-32. doi: 10.1128/aem.60.7.2524-2532.1994.
3
Ultrastructural and Immunocytochemical Studies on the H(2)O(2)-Producing Enzyme Pyranose Oxidase in Phanerochaete chrysosporium Grown under Liquid Culture Conditions.在液体培养条件下生长的糙皮侧耳中 H(2)O(2)产生酶吡喃糖氧化酶的超微结构和免疫细胞化学研究。
Appl Environ Microbiol. 1992 Nov;58(11):3667-76. doi: 10.1128/aem.58.11.3667-3676.1992.
4
Purification, characterization, and molecular cloning of a pyranose oxidase from the fruit body of the basidiomycete, Tricholoma matsutake.从担子菌松茸子实体中纯化、鉴定和分子克隆一种吡喃糖氧化酶
Biosci Biotechnol Biochem. 2003 Dec;67(12):2598-607. doi: 10.1271/bbb.67.2598.
5
Crystallization and preliminary X-ray diffraction analysis of pyranose 2-oxidase from the white-rot fungus Trametes multicolor.来自白腐真菌变色栓菌的吡喃糖2-氧化酶的结晶及初步X射线衍射分析
Acta Crystallogr D Biol Crystallogr. 2004 Jan;60(Pt 1):197-9. doi: 10.1107/s0907444903024922. Epub 2003 Dec 18.
6
Pyranose oxidase identified as a member of the GMC oxidoreductase family.
Bioinformatics. 2003 Jul 1;19(10):1216-20. doi: 10.1093/bioinformatics/btg140.
7
Identification of the covalent flavin adenine dinucleotide-binding region in pyranose 2-oxidase from Trametes multicolor.多色栓菌中吡喃糖2-氧化酶的共价黄素腺嘌呤二核苷酸结合区域的鉴定。
Anal Biochem. 2003 Mar 15;314(2):235-42. doi: 10.1016/s0003-2697(02)00661-9.
8
Transcript analysis of genes encoding a family 61 endoglucanase and a putative membrane-anchored family 9 glycosyl hydrolase from Phanerochaete chrysosporium.对来自黄孢原毛平革菌的编码61家族内切葡聚糖酶和一种假定的膜锚定9家族糖基水解酶的基因进行转录本分析。
Appl Environ Microbiol. 2002 Nov;68(11):5765-8. doi: 10.1128/AEM.68.11.5765-5768.2002.
9
Enzymatic synthesis of D-glucosone 6-phosphate (D-arabino-hexos-2-ulose 6-(dihydrogen phosphate)) and NMR analysis of its isomeric forms.D-葡萄糖酮6-磷酸酯（D-阿拉伯-己糖-2-酮糖6-（磷酸二氢酯））的酶促合成及其异构体形式的核磁共振分析。
Carbohydr Res. 2002 Sep 27;337(17):1585-7. doi: 10.1016/s0008-6215(02)00261-6.
10
Glyoxal oxidase of Phanerochaete chrysosporium: its characterization and activation by lignin peroxidase.黄孢原毛平革菌的乙二醛氧化酶：其特性及受木质素过氧化物酶的激活作用
Proc Natl Acad Sci U S A. 1990 Apr;87(8):2936-40. doi: 10.1073/pnas.87.8.2936.
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