Janse BJH, Gaskell J, Akhtar M, Cullen D
Department of Microbiology, University of Stellenbosch, Stellenbosch, 7600 South Africa.
Appl Environ Microbiol. 1998 Sep;64(9):3536-8. doi: 10.1128/AEM.64.9.3536-3538.1998.
Expression of Phanerochaete chrysosporium genes encoding ligninolytic enzymes was assessed in wood. Poly(A) RNA was extracted from colonized wood chips by magnetic capture, and specific transcripts were quantified by competitive reverse transcriptase PCR. mRNA levels varied substantially among lignin peroxidase genes, and transcript patterns were dramatically different from those in previous studies with defined media.
在木材中评估了编码木质素分解酶的黄孢原毛平革菌基因的表达。通过磁捕获从定殖的木屑中提取聚腺苷酸RNA,并通过竞争性逆转录酶PCR对特定转录本进行定量。木质素过氧化物酶基因之间的mRNA水平差异很大,并且转录模式与先前在特定培养基中的研究有显著不同。