Chetverina H V, Demidenko A A, Ugarov V I, Chetverin A B
Institute of Protein Research, Russian Academy of Sciences, Moscow Region.
FEBS Lett. 1999 Apr 30;450(1-2):89-94. doi: 10.1016/s0014-5793(99)00469-x.
The ability of RNAs to spontaneously rearrange their sequences under physiological conditions is demonstrated using the molecular colony technique, which allows single RNA molecules to be detected provided that they are amplifiable by the replicase of bacteriophage Qbeta. The rearrangements are Mg2+-dependent, sequence-non-specific, and occur both in trans and in cis at a rate of 10(-9) h(-1) per site. The results suggest that the mechanism of spontaneous RNA rearrangements differs from the transesterification reactions earlier observed in the presence of Qbeta replicase, and have a number of biologically important implications.
利用分子集落技术证明了RNA在生理条件下自发重排其序列的能力，该技术允许检测单个RNA分子，前提是它们可被噬菌体Qβ复制酶扩增。重排是Mg2+依赖性的、序列非特异性的，并且以每个位点10(-9) h(-1)的速率在反式和顺式中发生。结果表明，自发RNA重排的机制不同于早期在Qβ复制酶存在下观察到的酯交换反应，并且具有许多生物学上重要的意义。
