Morrison W D, Miller R V, Sayler G S
Appl Environ Microbiol. 1978 Nov;36(5):724-30. doi: 10.1128/aem.36.5.724-730.1978.
Transduction of Pseudomonas aeruginosa streptomycin resistance by a generalized transducing phage, F116, was shown to occur during a 10-day incubation in a flow-through environmental test chamber suspended in a freshwater reservoir. Mean F116 transduction frequencies ranged from 1.4 X 10(-5) to 8.3 X 10(-2) transductants per recipient during the in situ incubation. These transduction frequencies were comparable to transduction frequencies determined in preliminary laboratory transduction experiments. The results demonstrate the potential for naturally occurring transduction in aquatic environments and concurrent environmental and ecological ramifications.
一种广义转导噬菌体F116介导的铜绿假单胞菌链霉素抗性转导，在悬浮于淡水水库中的流通式环境试验箱中进行10天培养期间被证实会发生。原位培养期间，平均F116转导频率为每受体1.4×10⁻⁵至8.3×10⁻²个转导子。这些转导频率与初步实验室转导实验中确定的转导频率相当。结果证明了水生环境中自然发生转导的可能性以及随之而来的环境和生态影响。
