Ripp S, Ogunseitan O A, Miller R V
Department of Microbiology and Molecular Genetics, Oklahoma State University, Stillwater 74078.
Mol Ecol. 1994 Apr;3(2):121-6. doi: 10.1111/j.1365-294x.1994.tb00112.x.
A pseudolysogenic, generalized transducing bacteriophage, UT1, isolated from a natural freshwater habitat, is capable of mediating the transfer of both chromosomal and plasmid DNA between strains of Pseudomonas aeruginosa. Several chromosomal alleles from three different P. aeruginosa strains were found to transduce at frequencies from 10(-8) to 10(-10) transductants per PFU at multiplicities of infection (MOI) between 0.1 and 1. Transduction frequencies of certain alleles increased up to 1000-fold as MOIs were decreased to 0.01. UT1 is also capable of transducing plasmid DNA to indigenous populations of microorganisms in natural lake-water environments. Data obtained in this study suggest that environmentally endemic bacteriophages such as UT1 are formidable transducers of naturally occurring microbial communities. It should be possible to develop model systems to test transduction in freshwater environments using components derived exclusively from these environments.
从天然淡水生境中分离出的一种假溶原性、广义转导噬菌体UT1，能够介导铜绿假单胞菌菌株之间染色体DNA和质粒DNA的转移。在感染复数（MOI）介于0.1和1之间时，发现来自三种不同铜绿假单胞菌菌株的几个染色体等位基因的转导频率为每噬菌斑形成单位（PFU）产生10^(-8)至10^(-10)个转导子。当MOI降至0.01时，某些等位基因的转导频率增加高达1000倍。UT1还能够将质粒DNA转导至天然湖水环境中的本地微生物群体。本研究获得的数据表明，诸如UT1之类的环境中普遍存在的噬菌体是天然存在的微生物群落的强大转导者。利用完全源自这些环境的成分开发模型系统来测试淡水环境中的转导应该是可行的。
