Morgan A F
J Bacteriol. 1979 Jul;139(1):137-40. doi: 10.1128/jb.139.1.137-140.1979.
A mutant of the virulent bacteriophage E79 was isolated which mediated generalized transduction in Pseudomonas aeruginosa. Variable recovery of transductants as a result of phage killing was avoided by the use of recipients carrying the IncP-2 plasmid R38, and transduction frequencies of 4 X 10(-6) to 1 X 10(-5) per plaque-forming unit were obtained. Linkage studies have indicated that the coinheritance frequencies are less than would be expected from the published molecular weight of E79 deoxyribonucleic acid (120 X 10(6). By using recipients carrying R38, low-frequency transduction by wild-type E79 and two other virulent phages, F8 and phi 16, was demonstrated.
分离出了一种烈性噬菌体E79的突变体，它能在铜绿假单胞菌中介导普遍性转导。通过使用携带IncP-2质粒R38的受体菌，避免了由于噬菌体杀伤导致的转导子回收差异，获得了每噬菌斑形成单位4×10⁻⁶至1×10⁻⁵的转导频率。连锁研究表明，共遗传频率低于根据已发表的E79脱氧核糖核酸分子量（120×10⁶）所预期的值。通过使用携带R38的受体菌，证明了野生型E79以及另外两种烈性噬菌体F8和φ16的低频转导。
