人α和β珠蛋白信使核糖核酸的3'末端序列：与兔珠蛋白信使核糖核酸的比较

The 3' terminal sequences of human alpha and beta globin messenger RNAs: comparison with rabbit globin messenger RNA.

作者信息

Proudfoot N J, Longley J I

出版信息

Cell. 1976 Dec;9(4 PT 2):733-46. doi: 10.1016/0092-8674(76)90137-9.

Abstract

Sequences of 43 and 70 nucleotides adjacent to the 3' terminal poly(A) of human alpha and beta globin mRNAs have been established. Sequence analysis of complementary DNA from both normal and thalassemic globin mRNA preparations was carried out using endonuclease IV digestion and limited synthesis procedures. The two RNA sequences are 83% homologous to rabbit globin mRNA (Proudfoot, 1976), and a part of the alpha globin mRNA sequence codes for the carboxy terminus of human alpha globin Constant Spring (Clegg, Weatherall, and Milner, 1971). This latter result predicts that the 3' noncoding region of human alpha globin mRNA is exactly 112 nucleotides, while the 5' noncoding region is probably about 50 nucleotides.

摘要

已确定了与人α和β珠蛋白mRNA 3'末端多聚腺苷酸相邻的43和70个核苷酸的序列。使用核酸内切酶IV消化和有限合成程序对来自正常和地中海贫血珠蛋白mRNA制剂的互补DNA进行了序列分析。这两个RNA序列与兔珠蛋白mRNA的同源性为83%（普劳德富特，1976年），α珠蛋白mRNA序列的一部分编码人α珠蛋白恒春型的羧基末端（克莱格、韦瑟罗尔和米尔纳，1971年）。后一结果预测人α珠蛋白mRNA的3'非编码区正好是112个核苷酸，而5'非编码区可能约为50个核苷酸。