兔α-珠蛋白信使核糖核酸3'末端三分之一的核苷酸序列：与人类α-珠蛋白信使核糖核酸的比较。

Nucleotide sequence of the 3' terminal third of rabbit alpha-globin messenger RNA: comparison with human alpha-globin messenger RNA.

作者信息

Proudfoot N J, Gillam S, Smith M, Longley J I

出版信息

Cell. 1977 Aug;11(4):807-18. doi: 10.1016/0092-8674(77)90293-8.

Abstract

Rabbit and human alpha-globin complementary DNA was synthesized using the primer (dT10)dGdCdC hybridized to globin mRNA with reverse transcriptase, and sequenced using the plus and minus gel sequencing procedure (Sanger and Coulson, 1975; Brownlee and Cartwright, 1977). The complete 3' noncoding region, 89 nucleotides in length, and one third of the coding region of rabbit alpha-globin mRNA have thus been sequenced. These data are compared with the near complete 3' noncoding region sequence of human alpha-globin mRNA obtained in these and previous studies. The two mRNAs are approximately 80% homologous in their 3' noncoding regions, except that the human sequence has an insert of 24 nucleotides. A very similar insert in sequence has been described in human beta-globin mRNA (Proudfoot, 1977).

摘要

使用与珠蛋白mRNA杂交的引物（dT10）dGdCdC，通过逆转录酶合成兔和人的α-珠蛋白互补DNA，并采用正负链凝胶测序法进行测序（桑格和库尔森，1975年；布朗利和卡特赖特，1977年）。由此已对兔α-珠蛋白mRNA长度为89个核苷酸的完整3'非编码区以及三分之一的编码区进行了测序。将这些数据与在本研究及先前研究中获得的人α-珠蛋白mRNA的近乎完整的3'非编码区序列进行比较。两种mRNA在其3'非编码区约80%同源，只是人类序列有一个24个核苷酸的插入片段。人β-珠蛋白mRNA中也描述了一个序列非常相似的插入片段（普劳德富特，1977年）。