小鼠免疫球蛋白轻链信使核糖核酸3'非编码区的序列分析
Sequence analysis of the 3' non-coding region of mouse immunoglobulin light chain messenger RNA.
作者信息
Hamlyn P H, Gillam S, Smith M, Milstein C
出版信息
Nucleic Acids Res. 1977 Apr;4(4):1123-34. doi: 10.1093/nar/4.4.1123.
Abstract
Using an oligonucleotide d(pT10-C-A) as primer, cDNA has been transcribed from the 3' non-coding region of mouse immunoglobulin light chain mRNA and sequenced by a modification1 of the 'plus-minus' gel method2. The sequence obtained has partially corrected and extended a previously obtained sequence3. The new data contains an unusual sequence in which a trinucleotide is repeated seven times.
摘要
以寡核苷酸d(pT10 - C - A)为引物，从小鼠免疫球蛋白轻链mRNA的3'非编码区转录出cDNA，并通过对“加减法”凝胶法进行改进后进行测序。获得的序列部分校正并扩展了先前得到的序列。新数据包含一个异常序列，其中一个三核苷酸重复了七次。
Zotero 插件