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小鼠免疫球蛋白轻链信使核糖核酸3'非编码区的序列分析 - Suppr | 超能文献

小鼠免疫球蛋白轻链信使核糖核酸3'非编码区的序列分析

Sequence analysis of the 3' non-coding region of mouse immunoglobulin light chain messenger RNA.

作者信息

Hamlyn P H, Gillam S, Smith M, Milstein C

出版信息

Nucleic Acids Res. 1977 Apr;4(4):1123-34. doi: 10.1093/nar/4.4.1123.

DOI:10.1093/nar/4.4.1123

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC342509/

Abstract

Using an oligonucleotide d(pT10-C-A) as primer, cDNA has been transcribed from the 3' non-coding region of mouse immunoglobulin light chain mRNA and sequenced by a modification1 of the 'plus-minus' gel method2. The sequence obtained has partially corrected and extended a previously obtained sequence3. The new data contains an unusual sequence in which a trinucleotide is repeated seven times.

摘要

以寡核苷酸d(pT10 - C - A)为引物，从小鼠免疫球蛋白轻链mRNA的3'非编码区转录出cDNA，并通过对“加减法”凝胶法进行改进后进行测序。获得的序列部分校正并扩展了先前得到的序列。新数据包含一个异常序列，其中一个三核苷酸重复了七次。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b8f6/342509/0aa4921b8cb1/nar00477-0354-a.jpg

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b8f6/342509/0aa4921b8cb1/nar00477-0354-a.jpg

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b8f6/342509/0aa4921b8cb1/nar00477-0354-a.jpg

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本文引用的文献

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2

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Eur J Biochem. 1969 Dec;11(2):395-9. doi: 10.1111/j.1432-1033.1969.tb00786.x.

3

Enzymatic synthesis of deoxyribo-oligonucleotides of defined sequence. Properties of the enzyme.特定序列脱氧核糖寡核苷酸的酶促合成。该酶的性质。

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