Piasecka A, Marciniak K, Koter M, Krajewska E, Leyko W, Bryszewska M
Institute of Biophysics, University of Lódź, Poland.
Scand J Clin Lab Invest. 1999 Apr;59(2):147-52. doi: 10.1080/00365519950185887.
The effects of perindoprilat on the morphology and dynamic properties of human erythrocytes were studied by light microscopy, electron spin resonance spectroscopy and spectrophotometric methods. Erythrocytes were exposed to perindoprilat at 37 degrees C for 30 and 120 min. It was shown that the drug at a concentration of 0.75 microg/ml did not cause significant changes in the structure of erythrocyte membranes. Higher doses of the drug (7.5 and 75 microg/ml) induced changes in membrane fluidity in the hydrophobic core of the lipid bilayer, the conformation of membrane proteins, the number of SH groups and the activity of membrane-bound acetylcholinesterase (AChE). These modifications were accompanied by changes in the shape of erythrocytes and did not depend on time of incubation. Therefore, it is proposed that perindoprilat perturbs the lipid bilayer and disturbs the organization of the protein-lipid environment.
采用光学显微镜、电子自旋共振光谱法和分光光度法研究了培哚普利拉对人红细胞形态和动力学特性的影响。将红细胞在37℃下暴露于培哚普利拉30分钟和120分钟。结果表明，浓度为0.75微克/毫升的该药物不会引起红细胞膜结构的显著变化。更高剂量的药物（7.5和75微克/毫升）会诱导脂质双分子层疏水核心处的膜流动性、膜蛋白构象、SH基团数量以及膜结合乙酰胆碱酯酶（AChE）活性发生变化。这些改变伴随着红细胞形状的变化，且不依赖于孵育时间。因此，有人提出培哚普利拉会扰乱脂质双分子层并破坏蛋白质-脂质环境的组织。
