Soszyński M, Bartosz G
Department of Molecular Biophysics, University of Lódź, Poland.
Biochim Biophys Acta. 1996 Oct 24;1291(2):107-14. doi: 10.1016/0304-4165(96)00052-9.
The action of peroxynitrite on human erythrocytes and erythrocyte membranes was studied. Peroxynitrite (0.1-2 mM) induced a transient decrease of intracellular reduced glutathione, oxidized membrane protein -SH groups, initiated membrane lipid peroxidation and inactivated erythrocyte membrane acetylcholinesterase and ATPase activities. Membranes exposed to peroxynitrite showed aggregation and nitration of proteins and changes in protein organization detectable with a maleimide spin label.
研究了过氧亚硝酸盐对人红细胞及红细胞膜的作用。过氧亚硝酸盐（0.1 - 2 mM）可引起细胞内还原型谷胱甘肽短暂减少、膜蛋白 -SH 基团氧化、引发膜脂质过氧化，并使红细胞膜乙酰胆碱酯酶和 ATP 酶活性失活。暴露于过氧亚硝酸盐的膜显示出蛋白质聚集和硝化，以及用马来酰亚胺自旋标记可检测到的蛋白质组织变化。
