Piasecka A, Leyko W, Krajewska E, Bryszewska M
Department of General Biophysics, University of Lódź, Poland.
Scand J Clin Lab Invest. 2000 Aug;60(5):395-402. doi: 10.1080/003655100750019305.
Erythrocyte membrane fluidity, membrane potential and acetylcholinesterase activity were estimated after in vitro combined treatment of human erythrocytes with perindoprilat and low-power red light irradiation. Membrane fluidity was determined using fluorescent labels spectroscopy; membrane potential was evaluated by means of potential-sensitive fluorescent dyes; and acetylcholinesterase activity was estimated using the Ellman method. Both perindoprilat and laser irradiation, when used separately, increase microviscosity in the polar region and hyperpolarize the membranes in comparison with control erythrocytes. The combined action of these agents does not cause any further change in these parameters. Perindoprilat has an additional inhibitory effect on the activity of acetylcholinesterase, whereas laser irradiation causes an increase in the activity of the enzyme. Their combined action restores the initial activity of the enzyme independently of the sequence of treatment with both agents.
在培哚普利拉与低功率红光照射对人红细胞进行体外联合处理后，对红细胞膜流动性、膜电位和乙酰胆碱酯酶活性进行了评估。使用荧光标记光谱法测定膜流动性；通过电位敏感荧光染料评估膜电位；使用埃尔曼法估计乙酰胆碱酯酶活性。与对照红细胞相比，培哚普利拉和激光照射单独使用时，均会增加极性区域的微粘度并使膜超极化。这些药物的联合作用不会使这些参数发生任何进一步变化。培哚普利拉对乙酰胆碱酯酶的活性有额外的抑制作用，而激光照射会导致该酶活性增加。它们的联合作用可独立于两种药物的处理顺序恢复酶的初始活性。
