Essmail M, Baker D, Collins J, Vandewoude S, Salman M, Hegazy A A
Department of Pathology, Animal Reproduction Research Institute, El Haram, Giza, Egypt.
J Vet Diagn Invest. 1999 May;11(3):237-9. doi: 10.1177/104063879901100305.
A dot immunobinding assay (DIA) was used for the detection of antibody against bovine herpesvirus 4 (BHV-4) in experimentally infected specific-pathogen-free male and female rabbits. A semipurified virus preparation was used as the antigen, and protein A/G-horseradish-peroxidase conjugate and diaminobenzidine tetrahydrochloride were used as the detection system. Results of the DIA procedure on serum samples of experimentally infected male and female rabbits were compared with those of a complement-dependent virus neutralization (VN) test. None of the tested sera (0/60 samples) from either male or female rabbits were positive by the complement-dependent VN test. Results of the DIA procedure for the same tested sera were positive in 35 of 60 samples (58%) from BHV-4 infected rabbits, indicating higher sensitivity of DIA procedure as compared with the complement-dependent VN test.
采用斑点免疫结合试验（DIA）检测实验感染的无特定病原体的雄性和雌性兔血清中抗牛疱疹病毒4（BHV-4）抗体。使用半纯化病毒制剂作为抗原，蛋白A/G-辣根过氧化物酶结合物和盐酸二氨基联苯胺作为检测系统。将实验感染的雄性和雌性兔血清样本的DIA检测结果与补体依赖性病毒中和（VN）试验结果进行比较。通过补体依赖性VN试验，雄性和雌性兔的所有检测血清（0/60份样本）均为阴性。来自BHV-4感染兔的60份样本中，有35份（58%）的相同检测血清的DIA检测结果为阳性，表明DIA检测方法比补体依赖性VN试验具有更高的灵敏度。
