Naeem K, Goyal S M
Department of Veterinary Diagnostic Investigation, College of Veterinary Medicine, University of Minnesota, Saint Paul 55108.
J Vet Diagn Invest. 1990 Apr;2(2):107-10. doi: 10.1177/104063879000200204.
A dot immunobinding assay (DIA) was developed for the detection of antibody against bovid herpesvirus-4 (BHV-4) in bovine sera. A semipurified virus preparation was used as the antigen and an antispecies horseradish peroxidase-labeled IgG and diaminobenzidine were employed as the detection system. The sensitivity and specificity of the DIA were similar to those of indirect fluorescent antibody test, indicating the suitability of DIA as a rapid field test for the detection of anti-BHV-4 antibodies in cattle.
开发了一种斑点免疫结合试验（DIA），用于检测牛血清中抗牛疱疹病毒4型（BHV-4）的抗体。使用半纯化的病毒制剂作为抗原，以抗种属辣根过氧化物酶标记的IgG和二氨基联苯胺作为检测系统。DIA的敏感性和特异性与间接荧光抗体试验相似，表明DIA适合作为一种快速现场检测牛抗BHV-4抗体的方法。
