Tsai S J, Hutchinson L J, Zarkower A
Department of Veterinary Science, Pennsylvania State University, University Park, 16802.
Can J Vet Res. 1989 Oct;53(4):405-10.
A rapid, simple and inexpensive dot immunobinding assay (DIA) was evaluated for the serodiagnosis of paratuberculosis in cattle. The assay was performed on nitrocellulose strips which were dotted with purified protoplasmic antigen of Mycobacterium paratuberculosis. After incubation with test serum samples, the bound antibodies were detected using an enzyme-amplified immunostaining procedure. The efficacy of DIA as a screening test for paratuberculosis was compared to that of an enzyme-linked immunosorbent assay (ELISA), a modified agar gel immunodiffusion (mAGID) test, and an AGID test using 329 serum samples from cattle which were examined for M. paratuberculosis infection by a sensitive fecal culture technique. The DIA and ELISA had comparable results and both of the enzyme immunoassays had higher sensitivity than tests based on AGID. The sensitivity of all four tests was influenced by the intensity of fecal bacterial shedding. Preabsorption of sera with Mycobacterium phlei increased the sensitivity of both enzyme immunoassays. the specificity but reduced the sensitivity of both enzyme immunoassays.
对一种快速、简单且廉价的斑点免疫结合试验（DIA）进行了评估，用于牛副结核病的血清学诊断。该试验在硝酸纤维素条上进行，条上点有副结核分枝杆菌的纯化原生质抗原。与检测血清样本孵育后，使用酶放大免疫染色程序检测结合的抗体。使用329份牛血清样本，通过灵敏的粪便培养技术检测副结核分枝杆菌感染情况，将DIA作为副结核病筛查试验的效能与酶联免疫吸附试验（ELISA）、改良琼脂凝胶免疫扩散（mAGID）试验和琼脂凝胶免疫扩散（AGID）试验进行了比较。DIA和ELISA结果相当，两种酶免疫测定法的敏感性均高于基于AGID的试验。所有四项试验的敏感性均受粪便细菌排出强度的影响。用草分枝杆菌对血清进行预吸收可提高两种酶免疫测定法的敏感性，但降低了两种酶免疫测定法的特异性。
