Kac G, Bougnoux M E, Feuilhade De Chauvin M, Sene S, Derouin F
Laboratoire de Parasitologie-Mycologie, Hôpital Saint-Louis, 1 avenue Claude Vellefaux, 75475 Paris Cedex 10, France.
Br J Dermatol. 1999 May;140(5):839-44. doi: 10.1046/j.1365-2133.1999.02812.x.
Trichophyton mentagrophytes is a common cosmopolitan dermatophyte species composed of two varieties: T. mentagrophytes var. interdigitale (anthropophilic form) and T. mentagrophytes var. mentagrophytes (zoophilic form). We used a random amplified polymorphic DNA (RAPD) method to study the genetic diversity of 46 clinical isolates of the T. mentagrophytes complex collected from 38 patients with different geographical origins (Europe, Africa, South America). The T. mentagrophytes were isolated either from a unique lesion for 31 patients, including two patients living together, or from at least two sites for seven patients. Only one primer of 15 primers tested showed DNA polymorphism in the isolates, producing 23 distinct patterns belonging to three clusters. There was no specific cluster grouping isolates from the same geographical origin. The same pattern is shared by all the four T. mentagrophytes var. mentagrophytes and 13 of 42 T. mentagrophytes var. interdigitale. An identity of strains responsible for several lesions in seven individuals suggests an homogeneous T. mentagrophytes population in the case of multiple lesions. In contrast, the dissimilarity of two strains recovered from two patients living together argues against person-to-person transmission in that case. This study indicates that RAPD can be successfully applied to show genetic diversity among T. mentagrophytes isolates.
须癣毛癣菌是一种常见的世界性皮肤癣菌，由两个变种组成：指间型须癣毛癣菌（嗜人型）和须癣毛癣菌变种须癣毛癣菌（嗜兽型）。我们使用随机扩增多态性DNA（RAPD）方法研究了从38名不同地理来源（欧洲、非洲、南美洲）的患者中收集的46株须癣毛癣菌复合体临床分离株的遗传多样性。须癣毛癣菌分离自31名患者的单个皮损，其中包括两名住在一起的患者，或分离自7名患者的至少两个部位。在测试的15种引物中，只有一种引物在分离株中显示出DNA多态性，产生了属于三个簇的23种不同模式。没有特定的簇将来自相同地理来源的分离株分组。所有四种须癣毛癣菌变种须癣毛癣菌以及42种指间型须癣毛癣菌中的13种都具有相同的模式。在7名个体中导致多处皮损的菌株具有一致性，这表明在多处皮损的情况下须癣毛癣菌群体是同质的。相比之下，从两名住在一起的患者中分离出的两种菌株的差异表明在这种情况下不存在人际传播。这项研究表明，RAPD可以成功应用于显示须癣毛癣菌分离株之间的遗传多样性。
