Fernandes M A, Proença M T, Nogueira A J, Oliveira L M, Santiago B, Santana I, Oliveira C R
Departamento de Zoologia, Universidade de Coimbra, 3000, Coimbra, Portugal.
Biochim Biophys Acta. 1999 May 31;1454(1):89-96. doi: 10.1016/s0925-4439(99)00030-7.
The blood lipid composition (plasma, platelets and leukocytes), platelet membrane fluidity, apolipoproteins A and B in the plasma of AD patients and control subjects with distinct Apo E genotypes were investigated. No significant differences were found between the Apo E genotype and the cholesterol, phospholipids, triglycerides and Apo B levels in the plasma; cholesterol and phospholipids levels in platelet and leukocyte membranes; and platelet membrane fluidity of AD and control groups. However, the phospholipid levels in the leukocyte membranes of the control subgroup with the genotypes epsilon3/epsilon3 and epsilon3/epsilon4 and the AD subgroups with the genotypes epsilon2/epsilon3 and epsilon3/epsilon3, epsilon3/epsilon4 and epsilon4/epsilon4 were significantly lower than those observed in the control subgroup with the genotype epsilon2/epsilon3. Moreover, the cholesterol and phospholipid levels in the platelet membranes of the AD subgroup with the epsilon2 allele were significantly higher than those in the AD subgroup without the epsilon2 allele and the control subgroups with and without the epsilon2 allele. A strong correlation was found between cholesterol and phospholipids levels in the platelet membranes of the AD and control subgroups without the epsilon2 allele, but the residual cholesterol level in the platelet membranes of the AD subgroup was twice that observed in the control subgroup. Furthermore, the Apo A levels in the plasma of the AD subgroup with the epsilon3 allele were significantly lower than those observed in the AD subgroup without the epsilon3 allele and the control subgroup with the epsilon3 allele. The results are discussed in terms of involvement of lipid metabolism in the etiopathogenesis of AD.
对具有不同载脂蛋白E（Apo E）基因型的阿尔茨海默病（AD）患者和对照受试者的血脂成分（血浆、血小板和白细胞）、血小板膜流动性、血浆载脂蛋白A和B进行了研究。在AD组和对照组之间，Apo E基因型与血浆中的胆固醇、磷脂、甘油三酯和Apo B水平；血小板和白细胞膜中的胆固醇和磷脂水平；以及血小板膜流动性均未发现显著差异。然而，基因型为ε3/ε3和ε3/ε4的对照亚组以及基因型为ε2/ε3、ε3/ε3、ε3/ε4和ε4/ε4的AD亚组的白细胞膜中的磷脂水平显著低于基因型为ε2/ε3的对照亚组中观察到的水平。此外，具有ε2等位基因的AD亚组的血小板膜中的胆固醇和磷脂水平显著高于没有ε2等位基因的AD亚组以及具有和不具有ε2等位基因的对照亚组。在没有ε2等位基因的AD亚组和对照亚组的血小板膜中，胆固醇和磷脂水平之间存在强相关性，但AD亚组的血小板膜中的残余胆固醇水平是对照亚组中观察到水平的两倍。此外，具有ε3等位基因的AD亚组的血浆中的Apo A水平显著低于没有ε3等位基因的AD亚组以及具有ε3等位基因的对照亚组中观察到的水平。从脂质代谢参与AD发病机制方面对结果进行了讨论。
