Nunes E L, dos Santos K R, Mondino P J, Bastos M do C, Giambiagi-deMarval M
Instituto de Microbiologia Prof. Paulo de Góes, Universidade Federal do Rio de Janeiro, Brazil.
Diagn Microbiol Infect Dis. 1999 Jun;34(2):77-81. doi: 10.1016/s0732-8893(99)00021-8.
The presence of the ileS-2 gene, responsible for mupirocin resistance, in clinical isolates of methicillin-resistant Staphylococcus aureus was determined by multiplex polymerase chain reaction. Three pairs of primers were used, which yielded specific fragments of femA (encoding a unique feature of S. aureus), mecA (encoding resistance to methicillin) and ileS-2 genes. The multiplex polymerase chain reaction system is an easy and time-saving technique that, together with a rapid method for DNA extraction by boiling, may be incorporated as a routine analysis in clinical diagnostic laboratories.
通过多重聚合酶链反应测定耐甲氧西林金黄色葡萄球菌临床分离株中负责莫匹罗星耐药的ileS - 2基因的存在情况。使用了三对引物，它们产生了femA（编码金黄色葡萄球菌的独特特征）、mecA（编码对甲氧西林的耐药性）和ileS - 2基因的特异性片段。多重聚合酶链反应系统是一种简便且省时的技术，与通过煮沸快速提取DNA的方法一起，可作为临床诊断实验室的常规分析方法。
