Kpoda Dissinviel S, Ajayi Abraham, Somda Marius, Traore Oumar, Guessennd Nathalie, Ouattara Aboubakar S, Sangare Lassana, Traore Alfred S, Dosso Mireille
Laboratoire des Sciences Appliquées et Nutritionnelles (LabSAN), Centre de Recherche en Sciences Biologiques, Alimentaires et Nutritionnelles (CRSBAN), Université Ouaga 1 Pr Joseph KI-ZERBO, 03 BP 7021, Ouagadougou 03, Burkina Faso.
Laboratoire National de Santé Publique, 09 BP 24, Ouagadougou 09, Burkina Faso.
BMC Res Notes. 2018 Jul 13;11(1):471. doi: 10.1186/s13104-018-3581-5.
Resistance to antibiotics most especially third generation cephalosporins has assumed a worrisome dimension globally. Genes conferring these resistance which are mediated by enzymes known as extended spectrum beta-lactamases (ESBLs) are now wide spread among several Enterobacteriaceae species. However there is paucity of data regarding the distribution of these genes in Burkina Faso. Hence this prospective study aims to determine the prevalence and distribution of ESBL encoding genes in ESBL producing Enterobacteriaceae strains isolated from clinical samples of patients attending the three major hospitals in Ouagadougou Burkina Faso.
ESBL-encoding genes were assayed in 187 ESBL producing Enterobacteriaceae strains. Among these isolates, the prevalence of ESBL-producing strains with blaTEM, blaSHV and blaCTX-M genes were 26.2% (49/187), 5.9% (11/187) and 40.1% (75/187) respectively. The association of ESBL encoding genes with health centers was statistically significant (p = 0.0209). Approximately 39.6% of E. coli harbored CTX-M and Klebsiella spp. 5.9%. This study demonstrates the dissemination of TEM, SHV and CTX-M genes in ESBL producing Enterobacteriaceae strains in Ouagadougou. Continuous spread of these bacteria poses great public health risk, thus increased surveillance and regulation of antibiotics use is imperative in Burkina Faso.
对抗生素尤其是第三代头孢菌素的耐药性在全球已成为一个令人担忧的问题。赋予这些耐药性的基因由称为超广谱β-内酰胺酶（ESBLs）的酶介导，目前在几种肠杆菌科细菌中广泛传播。然而，关于这些基因在布基纳法索的分布数据却很匮乏。因此，这项前瞻性研究旨在确定从布基纳法索瓦加杜古三大医院患者临床样本中分离出的产ESBL肠杆菌科菌株中ESBL编码基因的流行情况和分布。
对187株产ESBL的肠杆菌科菌株进行了ESBL编码基因检测。在这些分离株中，携带blaTEM、blaSHV和blaCTX - M基因的产ESBL菌株的流行率分别为26.2%（49/187）、5.9%（11/187）和40.1%（75/187）。ESBL编码基因与医疗中心之间的关联具有统计学意义（p = 0.0209）。约39.6%的大肠杆菌携带CTX - M基因，克雷伯菌属为5.9%。本研究证明了TEM、SHV和CTX - M基因在瓦加杜古产ESBL的肠杆菌科菌株中的传播。这些细菌的持续传播构成了巨大的公共卫生风险，因此布基纳法索必须加强对抗生素使用的监测和监管。
