Louie M, Read S, Louie L, Ziebell K, Rahn K, Borczyk A, Lior H
Department of Microbiology, SD Laboratory Services, Sunnybrook Health Science Centre, Toronto, Ontario, Canada.
Epidemiol Infect. 1999 Aug;123(1):17-24. doi: 10.1017/s0950268899002551.
The utility of phage typing, pulsed-field gel electrophoresis (PFGE), and plasmid profile analysis was compared, to differentiate between Canadian Escherichia coli O157:H7 strains of human (n = 27) and cattle (n = 24) origin. The diversity indices for phage typing, plasmid analysis and PFGE were 0.85, 0.69 and 0.93, respectively. PFGE and phage typing were also applied to study the role of direct transmission of E. coli O157:H7 from cattle to humans on isolates collected from two separate farm outbreaks. PFGE showed that more than one E. coli O157:H7 strain with varying PFGE DNA subtype profiles, may be responsible for an outbreak, and that more than one E. coli O157:H7 subtype may be circulating on a particular farm at any one time. To our knowledge, this is one of the first reports where PFGE typing was used to verify the direct transmission of E. coli O157:H7 from cattle to humans.
对噬菌体分型、脉冲场凝胶电泳（PFGE）和质粒图谱分析的效用进行了比较，以区分源自人类（n = 27）和牛（n = 24）的加拿大大肠杆菌O157:H7菌株。噬菌体分型、质粒分析和PFGE的多样性指数分别为0.85、0.69和0.93。PFGE和噬菌体分型还用于研究从两个独立农场疫情中分离出的大肠杆菌O157:H7从牛直接传播给人类的作用。PFGE表明，不止一种具有不同PFGE DNA亚型图谱的大肠杆菌O157:H7菌株可能导致一次疫情爆发，并且在任何特定时间，不止一种大肠杆菌O157:H7亚型可能在某个特定农场中传播。据我们所知，这是最早使用PFGE分型来验证大肠杆菌O157:H7从牛直接传播给人类的报告之一。
