利用海马体切片研究衰老如何改变脑组织中的离子调节。

Using hippocampal slices to study how aging alters ion regulation in brain tissue.

作者信息

Roberts E L

机构信息

Geriatric Research, Education, and Clinical Center, Miami VA Medical Center, Miami, Florida 33125, USA.

出版信息

Methods. 1999 Jun;18(2):150-9. doi: 10.1006/meth.1999.0768.

DOI:10.1006/meth.1999.0768

PMID:10356345

Abstract

Aging alters ion regulation in brain tissue. This article describes methods useful for studying such age-related changes in the rat hippocampal slice preparation. Topics considered include (a) selection of appropriate age groups of rats for aging studies, (b) a description of methods for preparing and maintaining hippocampal slices, (c) measurement of intracellular pH with the H+-sensitive dye carboxy-SNARF-1, and (d) measurement of extracellular pH and K+ with cation-selective microelectrodes.

摘要

衰老会改变脑组织中的离子调节。本文描述了一些有助于研究大鼠海马切片制备中此类与年龄相关变化的方法。所讨论的主题包括：(a) 为衰老研究选择合适年龄组的大鼠；(b) 海马切片制备和维持方法的描述；(c) 用H⁺敏感染料羧基-SNARF-1测量细胞内pH；以及(d) 用阳离子选择性微电极测量细胞外pH和K⁺。