用于测定蘑菇中痕量金属的微波消解方法的验证
Validation of microwave digestion method for determination of trace metals in mushrooms.
作者信息
Kucak A, Blanusa M
机构信息
Mining and Chemistry School, Varazdin, Croatia.
出版信息
Arh Hig Rada Toksikol. 1998 Dec;49(4):335-42.
A microwave digestion method for mushrooms, developed in the study, allows fast preparation of samples and reduces the contamination risk in the process of determining trace metals. Concentrations of six trace elements, Fe, Mn, Cu, Zn, Pb, and Cd were measured in 50 samples of different species of edible mushrooms (fam. Boletaceae) using atomic absorption spectrometry after microwave and dry ashing procedure. The methods were validated through certified Standard Reference Material SRM 1577b (Bovine Liver) which was treated and analysed using the same procedures as for the mushrooms. The samples were either digested with concentrated HNO3 in closed Teflon PFA vessels in a microwave oven, or ashed in quartz crucibles at 450 degrees C. The respective recoveries of Fe, Mn, Zn, Cu, Pb, and Cd obtained by measuring SRM were 112, 107, 104, 115, 111 and 95% after microwave digestion procedure and 86, 101, 109, 111, 98, and 110% after dry ashing procedure. The correlation between concentrations obtained by the two different methods of sample preparation was high for all metals.
本研究开发的一种用于蘑菇的微波消解方法，能够快速制备样品，并降低痕量金属测定过程中的污染风险。采用微波消解和干灰化程序后，使用原子吸收光谱法对50个不同种类的可食用蘑菇（牛肝菌科）样品中的六种微量元素（铁、锰、铜、锌、铅和镉）的浓度进行了测定。通过使用与蘑菇相同的程序处理和分析的有证标准参考物质SRM 1577b（牛肝）对方法进行了验证。样品要么在微波炉中于密闭的聚四氟乙烯全氟烷氧基容器中用浓硝酸消解，要么在石英坩埚中于450℃灰化。通过测量SRM获得的铁、锰、锌、铜、铅和镉在微波消解程序后的回收率分别为112%、107%、104%、115%、111%和95%，在干灰化程序后的回收率分别为86%、101%、109%、111%、98%和110%。两种不同样品制备方法所获得的浓度之间对于所有金属的相关性都很高。
Zotero 插件