Bandyopadhyay S K, Kataria R S, Tiwari A K
National Biotechnology Centre, Indian Veterinary Research Institute, Izatnagar, India.
Indian J Exp Biol. 1998 Oct;36(10):1034-7.
Reverse transcription-PCR (RT-PCR) technique was adopted to amplify a 101 basepair nucleotide sequence of bluetongue virus (BTV) genome segment 6. The specificity of the amplicon was determined by its approximate size in 3% agarose gel electrophoresis, digestion with restriction enzyme MspI, dot-blot hybridization and cycle sequencing. The technique was found to be suitable for detection of bluetongue virus in infected cell culture and clinical samples.
采用逆转录聚合酶链反应（RT-PCR）技术扩增蓝舌病毒（BTV）基因组第6片段的101个碱基对的核苷酸序列。扩增产物的特异性通过其在3%琼脂糖凝胶电泳中的大致大小、用限制性内切酶MspI消化、斑点杂交和循环测序来确定。结果发现该技术适用于检测感染细胞培养物和临床样本中的蓝舌病毒。
