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六种商业检测方法及芽管试验用于白色念珠菌初步鉴定的评估。

Evaluation of six commercial tests and the germ-tube test for presumptive identification of Candida albicans.

作者信息

Hoppe J E, Frey P

机构信息

University Children's Hospital, Tübingen, Germany.

出版信息

Eur J Clin Microbiol Infect Dis. 1999 Mar;18(3):188-91. doi: 10.1007/s100960050256.

Abstract

Six commercial tests (Albicans ID, bioMérieux, France; CandiSelect, Sanofi Diagnostics Pasteur, France; CHROMagar Candida, Becton Dickinson, USA; Fluoroplate Candida, Merck, Germany; Fongiscreen 4H, Sanofi Diagnostics Pasteur; and Murex Candida albicans, Murex Diagnostics, USA) and the germ-tube test for presumptive identification of Candida albicans were evaluated using clinical isolates of Candida albicans (n = 350) and of non-albicans yeasts (n = 135). Sensitivities and specificities of all tests regarding the identification of Candida albicans were greater than 97%. The two tests that also allow presumptive identification of some non-albicans yeasts (CHROMagar Candida and Fongiscreen 4H) did not identify all isolates of Candida glabrata and Candida tropicalis. In addition, CHROMagar Candida identified as Candida glabrata some isolates belonging to different species of non-albicans yeasts.

摘要

使用白色念珠菌临床分离株(n = 350)和非白色念珠菌酵母临床分离株(n = 135),对六种商业检测方法(法国生物梅里埃公司的白色念珠菌鉴定试剂盒;法国赛诺菲巴斯德诊断公司的念珠菌选择试剂盒;美国BD公司的CHROMagar念珠菌显色培养基;德国默克公司的荧光板念珠菌检测试剂盒;赛诺菲巴斯德诊断公司的Fongiscreen 4H试剂盒;美国Murex诊断公司的Murex白色念珠菌检测试剂盒)以及用于白色念珠菌初步鉴定的芽管试验进行了评估。所有检测方法针对白色念珠菌鉴定的敏感性和特异性均大于97%。另外两种还可对一些非白色念珠菌酵母进行初步鉴定的检测方法(CHROMagar念珠菌显色培养基和Fongiscreen 4H试剂盒)未能鉴定出所有光滑念珠菌和热带念珠菌分离株。此外,CHROMagar念珠菌显色培养基将一些属于不同非白色念珠菌酵母菌种的分离株鉴定为光滑念珠菌。

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