干扰素α-2b对掌腱膜挛缩体外模型的作用。

The effect of interferon-alpha2b on an in vitro model Dupuytren's contracture.

作者信息

Sanders J L, Dodd C, Ghahary A, Scott P G, Tredget E E

机构信息

Department of Surgery, University of Alberta, Edmonton, Canada.

出版信息

J Hand Surg Am. 1999 May;24(3):578-85. doi: 10.1053/jhsu.1999.0578.

DOI:10.1053/jhsu.1999.0578

PMID:10357539

Abstract

The effects of interferon-alpha2b (IFN-alpha2b) on Dupuytren's and control palmar fibroblasts were evaluated using the fibroblast-populated collagen lattice model. Three paired strains of Dupuytren's and control fibroblasts were exposed to IFN-alpha2b for 96 hours before incorporation into triplicate collagen lattices. Contraction of the lattices was recorded and Northern blot analysis of cytoskeletal mRNA was performed. Comparisons of Dupuytren's and control fibroblasts revealed significantly increased contractility of the Dupuytren's fibroblasts in 2 of the 3 strains. Treatment with IFN-alpha2b significantly inhibited contraction in both Dupuytren's and control fibroblasts. In Dupuytren's fibroblasts, treatment with IFN-alpha2b significantly down-regulated mRNA expression for cytoplasmic beta-actin and gamma-actin.

摘要

使用成纤维细胞填充的胶原晶格模型评估了干扰素α2b（IFN-α2b）对掌腱膜和对照掌部成纤维细胞的作用。在将三对掌腱膜和对照成纤维细胞系掺入一式三份的胶原晶格之前，将其暴露于IFN-α2b 96小时。记录晶格的收缩情况，并对细胞骨架mRNA进行Northern印迹分析。掌腱膜和对照成纤维细胞的比较显示，在3个细胞系中的2个中，掌腱膜成纤维细胞的收缩性显著增加。IFN-α2b处理显著抑制了掌腱膜和对照成纤维细胞的收缩。在掌腱膜成纤维细胞中，IFN-α2b处理显著下调了细胞质β-肌动蛋白和γ-肌动蛋白的mRNA表达。