蛋白质脉冲式喂养可改善老年女性的蛋白质保留情况。

Protein pulse feeding improves protein retention in elderly women.

作者信息

Arnal M A, Mosoni L, Boirie Y, Houlier M L, Morin L, Verdier E, Ritz P, Antoine J M, Prugnaud J, Beaufrère B, Mirand P P

机构信息

Unité d'Etude du Métabolisme Azoté, Institut National de la Recherche Agronomique, Clermont-Ferrand-Theix, France.

出版信息

Am J Clin Nutr. 1999 Jun;69(6):1202-8. doi: 10.1093/ajcn/69.6.1202.

DOI:10.1093/ajcn/69.6.1202

PMID:10357740

Abstract

BACKGROUND

Adequate protein nutrition could be used to limit gradual body protein loss and improve protein anabolism in the elderly.

OBJECTIVE

We tested the hypothesis that an uneven protein feeding pattern was more efficient in improving protein anabolism than was an even pattern.

DESIGN

After a controlled period, 15 elderly women (mean age: 68 y) were fed for 14 d either a pulse diet (n = 7), providing 80% of the daily protein intake at 1200, or a spread diet (n = 8), in which the same daily protein intake was spread over 4 meals. Both diets provided 1.7 g protein x kg fat-free mass (FFM)(-1) x d(-1). Protein accretion and daily protein turnover were determined by using the nitrogen balance method and the end product method (ammonia and urea) after an oral dose of [15N]glycine.

RESULTS

Nitrogen balance was more positive with the pulse than with the spread diet (54 +/- 7 compared with 27 +/- 6 mg N x kg FFM(-1) x d(-1); P < 0.05). Protein turnover rates were also higher with the pulse than with the spread diet (5.58 +/- 0.22 compared with 4.98 +/- 0.17 g protein x kg FFM(-1) x d(-1); P < 0.05), mainly because of higher protein synthesis in the pulse group (4.48 +/- 0.19 g protein x kg FFM(-1) x d(-1)) than in the spread group (3.75 +/- 0.19 g protein x kg FFM(-1) x d(-1)) (P < 0.05).

CONCLUSION

A protein pulse-feeding pattern was more efficient than was a protein spread-feeding pattern in improving, after 14 d, whole-body protein retention in elderly women.

摘要

背景

充足的蛋白质营养可用于限制老年人身体蛋白质的逐渐流失并改善蛋白质合成代谢。

目的

我们检验了这样一种假设，即不均衡的蛋白质喂养模式在改善蛋白质合成代谢方面比均衡模式更有效。

设计

经过一段对照期后，15名老年女性（平均年龄：68岁）被给予14天的饮食，其中7名采用脉冲式饮食（每日蛋白质摄入量的80%于12点摄入），8名采用分散式饮食（相同的每日蛋白质摄入量分散在4餐中）。两种饮食均提供1.7克蛋白质×千克去脂体重（FFM）⁻¹×天⁻¹。口服[¹⁵N]甘氨酸后，采用氮平衡法和终产物法（氨和尿素）测定蛋白质蓄积和每日蛋白质周转率。

结果

脉冲式饮食组的氮平衡比分散式饮食组更正向（分别为54±7与27±6毫克氮×千克FFM⁻¹×天⁻¹；P<0.05）。脉冲式饮食组的蛋白质周转率也高于分散式饮食组（分别为5.58±0.22与4.98±0.17克蛋白质×千克FFM⁻¹×天⁻¹；P<0.05），主要是因为脉冲组的蛋白质合成（4.48±0.19克蛋白质×千克FFM⁻¹×天⁻¹）高于分散组（3.75±0.19克蛋白质×千克FFM⁻¹×天⁻¹）（P<0.05）。