Moriuchi H, Moriuchi M, Fauci A S
Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892, USA.
J Immunol. 1999 Jun 15;162(12):7543-8.
Allogeneic stimulation presents an immunologic challenge during pregnancy, blood transfusions, and transplantations, and has been associated with reactivation of latently infected virus such as CMV. Since HIV-1 is transmitted vertically, sexually, or via contaminated blood, we have tested the effects of allostimulation on HIV-1 infection. 1) We show that allostimulated lymphocytes are highly susceptible to acute infection with T cell-tropic or dual-tropic HIV-1. 2) We show that allostimulation has dichotomous effects on replication of macrophage-tropic HIV-1; it activates HIV expression in already infected cells but inhibits HIV entry by secreting HIV-suppressive CC chemokines. 3) We show that allogeneic stimulation of latently infected, resting CD4+ T cells induced replication of HIV-1 in these cells. These observations suggest that allogeneic stimulation may play a role in the transmission, replication, and phenotypic transition of HIV-1.
同种异体刺激在妊娠、输血和移植过程中会带来免疫挑战，并且与潜伏感染病毒（如巨细胞病毒）的重新激活有关。由于HIV-1可通过垂直传播、性传播或经污染血液传播，我们检测了同种异体刺激对HIV-1感染的影响。1）我们发现，同种异体刺激的淋巴细胞对嗜T细胞或双嗜性HIV-1的急性感染高度敏感。2）我们发现，同种异体刺激对嗜巨噬细胞HIV-1的复制具有双重作用；它激活已感染细胞中的HIV表达，但通过分泌抑制HIV的CC趋化因子来抑制HIV的进入。3）我们发现，对潜伏感染的静息CD4+T细胞进行同种异体刺激可诱导这些细胞中HIV-1的复制。这些观察结果表明，同种异体刺激可能在HIV-1的传播、复制和表型转变中起作用。
