Kanda K, Kitajima Y, Moriyama Y, Kato F, Murata A
Department of Applied Biological Sciences, Saga University, Japan.
Acta Virol. 1998 Nov;42(5):315-8.
A region homologous to the genome of plasmid integrative phage J7W-1 was detected in the largest plasmid in 3 out of 22 type strains of Bacillus thuringiensis, dendrolimus (DEN), aizawai (AIZ) and indiana (IND). Phage induction by ethidium bromide observed particularly in the J7W-1 lysogen was identified in DEN and IND but not AIZ strains. The morphology of the phage induced in DEN and IND strains was identical to J7W-1, but the phage production in IND strain was lower as compared to the J7W-1 lysogen. Although the restriction analysis indicated that the prophage in DEN strain possessed a complete J7W-1 genome, modification and/or deletion had presumably occurred in AIZ and IND strains.
在苏云金芽孢杆菌22株模式菌株中的3株，即松毛虫（DEN）、阿维菌素（AIZ）和印第安纳（IND）的最大质粒中，检测到了与质粒整合噬菌体J7W-1基因组同源的区域。在DEN和IND菌株中发现了溴化乙锭诱导噬菌体现象，特别是在J7W-1溶原菌中观察到的这种现象，但在AIZ菌株中未发现。在DEN和IND菌株中诱导出的噬菌体形态与J7W-1相同，但与J7W-1溶原菌相比，IND菌株中的噬菌体产量较低。尽管限制性分析表明DEN菌株中的原噬菌体拥有完整的J7W-1基因组，但AIZ和IND菌株中可能发生了修饰和/或缺失。
