苏云金芽孢杆菌型菌株中存在溶原性噬菌体。

Existence of lysogenic bacteriophages in Bacillus thuringiensis type strains.

机构信息

Department of Agricultural Biotechnology, College of Agriculture & Life Sciences, Seoul National University, Seoul 151-742, Republic of Korea.

出版信息

J Invertebr Pathol. 2013 Jul;113(3):228-31. doi: 10.1016/j.jip.2013.04.008. Epub 2013 Apr 28.

DOI:10.1016/j.jip.2013.04.008

PMID:23632013

Abstract

We screened the existence of bacteriophages in 67 Bacillus thuringiensis type strains by phage DNA extraction and PCR using phage terminase small subunit (TerS)-specific primers to the supernatants and the precipitated pellets of Bt cultures, and by transmission electron microscopy. The various bacteriophages were observed from the supernatants of 22 type strains. Ten type strains showed the extracted phage DNAs and the amplified fragment by TerS PCR but 12 type strains showed only the phage DNAs. Their morphological characteristic suggests that they belong to Family Siphoviridae which had a long tail and symmetrical head.

摘要

我们通过噬菌体 DNA 提取和 PCR 筛选了 67 株苏云金芽孢杆菌（Bacillus thuringiensis）模式菌株中噬菌体的存在情况，使用噬菌体终止酶小亚基（TerS）特异性引物对 Bt 培养物的上清液和沉淀颗粒进行了检测，同时还使用了透射电子显微镜。从 22 株模式菌株的上清液中观察到了各种噬菌体。10 株模式菌株显示出了可提取的噬菌体 DNA 和 TerS PCR 扩增片段，但 12 株模式菌株仅显示出了噬菌体 DNA。它们的形态特征表明它们属于长尾和对称头部的肌尾病毒科（Family Siphoviridae）。